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Optimization of factors affecting Agrobacterium-mediated hairy root induction in Vitex negundo L. (Lamiaceae) Skip to content Yıl 2024, Cilt: 11 Sayı: 2, 244 – 254, 03.06.2024
Bhaswatımayee Mahakur Arpıta Moharana Sanjay Kumar Madkamı Soumendra Kumar Naık Durga Prasad Barık
https://doi.org/10.21448/ijsm.1368677
Öz Vitex negundo L. is an aromatic, woody, blooming shrub in the Lamiaceae family which can grow into a small tree. Traditionally, V. negundo root has been used to cure diabetes, colic, boils, leprosy, and rheumatism. Keeping the importance of its roots in mind, an attempt has been taken for development of a protocol for efficient hairy root proliferation system. The Agrobacterium rhizogenes strains (MTCC 532 and MTCC 2364) were used for hairy root induction. For A. rhizogenes infection, both in vitro and in vivo leaves as well as internodes were used as explant. In vitro leaves and internodal explants were obtained by the inoculation of matured nodal segments on the optimum medium [MS + 2.0 mg/L N6-Benzylaminopurine (BAP)] with c.a. 91.6% shoot regeneration and an average of 8.1 shoots per explants. In vitro leaf showed best hairy root induction followed by in vitro internode on ½ MS medium augmented with acetosyringone. Highest transformation efficiency was achieved using MTCC 2364 strain, while no transformation was observed in MTCC 532 strain. Different factors affecting transformation including co-cultivation period, infection time and optical density (O.D.) value were standardized. The highest efficacy, 88.8% hairy root induction was observed in in vitro leaves infected by MTCC 2364 for 60 minutes infection time with an O.D. value of 0.29 maintained over a 44-48 hours of co-cultivation period. The prescribed protocol may be used as a reference for development of industrial scale hairy root production for bioactive compound located in root of V. negundo.
Anahtar Kelimeler Acetosyringone, A. rhizogenes, Co-cultivation period, Hairy root culture, Vitex negundo
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Effect of different Agrobacterium rhizogenes strains for in-vitro hairy root induction, totalphenolic, flavonoids contents, antibacterial and antioxidant activity of (Cucumis anguria L.). Saudi Journal of Biological Sciences, 27(11), 2972–2979. http://dx.doi.org/10.1016/j.sjbs.2020.08.050 Ji, H., Yang, B., Jing, Y., Luo, Y., Li, B., & Yan, Y. (2023). Trehalose and brassinolide enhance the signature ingredient accumulation and anti-oxidant activity in the hairy root cultures of Polygala tenuifolia Wild. Industrial Crops and Products, 196, 116521. https://doi.org/10.1016/j.indcrop.2023.116521 Kamal, N., Asni, N.S.M., Rozlan, I.N.A., Azmi, M.A.H.M., Mazlan, N.W., Mediani, A., Baharum, S.N., Latip, J., Assaw, S., & Edrada-Ebel, R.A. (2022). Traditional Medicinal Uses, Phytochemistry, Biological Properties, and Health Applications of Vitex sp. Plants, 11(15), 1944. http://dx.doi.org/10.3390/plants11151944 Kumar, A., Kumari, A., Demiwal, P., Roy, P., & Sircar, D. (2023). Enhanced production of bioactive plumbagin in hairy root cultures and adventitious root cultures of Plumbago zylanica L. by a novel apocarotenoid elicitor, α-ionone. Industrial Crops and Products, 203, 117140. https://doi.org/10.1016/j.indcrop.2023.117140 Kumar, V., Sharma, A., Prasad, B.C.N., Gururaj, H.B., & Ravishankar, G.A. (2006). Agrobacterium rhizogenes mediated genetic transformation resulting in hairy root formation is enhanced by ultrasonication and acetosyringone treatment. Electronic Journal of Biotechology, 9(4), 349–357. Ladda, P.L., & Magdum, C.S. (2012). Vitex negundo Linn.: ethnobotany, phytochemistry and pharmacology- a review. International Journal of Advances in Pharmacy Biology and Chemistry, 1(1), 111-120. Mahendran, G., Verma, N., Singh, M., Shanker, K., Banerjee, S., Kumar, B., Rahman, L. (2022). Elicitation enhances swerchirin and 1, 2, 5, 6-tetrahydroxyxanthone production in hairy root cultures of Swertia chirayita (Roxb.) H. Karst. Industrial Crops and Products, 177, 114488. http://dx.doi.org/10.1016/j.indcrop.2021.114488 Majumdar, S., Garai, S., Jha, S. (2011). Genetic transformation of Bacopa monnieri by wild type strains of Agrobacterium rhizogenes stimulates production of Bacopa saponins in transformed calli and plants. Plant Cell Reports, 30, 941 954. http://dx.doi.org/10.1007/s00299-011-1035-9 Moola, A.K., Thiruppathi, S.K., Ranjitha Kumari, B.D. (2021). Enhancement of Celastrol compound by silver nanoparticles and acetosyringone in Celastrus paniculatus Wild. through adventitious and hairy root culture. Journal of plant biochemistry and biotechnology, 31(4). http://dx.doi.org/10.1007/s13562-021-00676-y Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473 497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x Muthiah, J.V.L., Lakkakula, S., Rajaiah, J., Jayabalan, S., & Manikandan, R. (2016). Analysis of propagation of Bacopa monnieri (L.) from hairy roots, elicitation and Bacoside A contents of Ri transformed plants. World Journal of Microbiology and Biotechnology, 32,131. https://doi.org/10.1007/s11274-016-2083-7 Nadkarni, K.M. (2002). Indian Materia Medica, Bombay Popular Prakashan, (vol 1), 1278-1280. Orlikowska, T.K., Cranston, H.J., Dyer, W.E. (1995). Factors influencing Agrobacterium tumefaciens mediated transformation and regeneration of the safflower cultivar ‘Centennial’. Plant Cell Tissue and Organ Culture, 40, 85-91. Rana, S., & Rana, K.K. (2014). Review on medicinal usefulness of Vitex negundo Linn. Open Access Library Journal, 1(3), 1-13. http://dx.doi.org/10.4236/oalib.1100508 Sahu, L., Jena S., Swain S.S., Sahoo, S., Chand, P.K. (2013). Agrobacterium rhizogenes-mediated transformation of a multi-medicinal herb, Boerhaavia diffusa L.: optimization of the process and anti-microbial activity against bacterial pathogens causing urinary tract infections. Frontiers in Life Science, 7(3 4), 197 209. https://doi.org/10.1080/21553769.2013.879266 Sajjalaguddam, R., & Paladugu, A. (2016). Influence of Agrobacterium rhizogenes strains and elicitation on hairy root induction and Glycyrrhizin production from Abrus precatorius. Journal of Pharma ceutical Sciences and Research, 8(12), 1353-1357. Shahabzadeh, Z., Heidari, B., Hafez, R.F. (2014). Induction of transgenic hairy roots in Trigonella foenum-graceum co-cultivated with Agrobacterium rhizogenes harboring a GFP gene. Journal of Crop Science and Biotechnology, 16, 263 268. https://doi.org/10.1007/s12892-013-0082-x Singh, K.S., Frdrik, P.G., Ramamurthy, M. (2016). Phytochemical and antibacterial activity of Vitex negundo, Leucasaspera and Abutilon indicum against wound pathogens. International Journal of Current Research in Biology and Medicines, 1, 60-90. Srinivasan, R., Kamalanathan, D., Boobalan, S., Saranyaa, V., Mouliganesh, S., & Seenivasagan, R. (2023). Agrobacterium rhizogenes influences aervine enhancement in hairy root culture of Aerva javanica (Burm.f.) Juss. ex Schult and in silico assessment of human breast cancer activity. Journal of Applied Biology and Biotechnology, 11(4), 148-158. http://dx.doi.org/10.7324/JABB.2023.23350 Swain, S.S., Sahu, L., Barik, D.P., & Chand, P.K. (2010). Agrobacterium × plant factors influencing transformation of ‘Joseph’s coat’ (Amaranthus tricolor L.). Scientia Horticulturae, 125(3), 461-468. .http://dx.doi.org/10.1016/j.scienta.2010.04.034 Usha, P.K., Benjamin, S., Mohanan, K.V., & Raghu, A.V. (2007). An efficient micropropagation system for Vitex negundo L., an important woody aromatic medicinal plant, through shoot tip culture. Research Journal of Botany, 2, 102 107. http://dx.doi.org/10.3923/rjb.2007.102.107 Yunos, N.M., Kean, O.B., Abas, R. (2005). Cytotoxicity evaluations on Vitex negundo anti-inflammatory extracts. Malaysian Journal of Science, 24(1), 213-217.
Optimization of factors affecting Agrobacterium-mediated hairy root induction in Vitex negundo L. (Lamiaceae) Yıl 2024, Cilt: 11 Sayı: 2, 244 – 254, 03.06.2024
Bhaswatımayee Mahakur Arpıta Moharana Sanjay Kumar Madkamı Soumendra Kumar Naık Durga Prasad Barık
https://doi.org/10.21448/ijsm.1368677
Öz Vitex negundo L. is an aromatic, woody, blooming shrub in the Lamiaceae family which can grow into a small tree. Traditionally, V. negundo root has been used to cure diabetes, colic, boils, leprosy, and rheumatism. Keeping the importance of its roots in mind, an attempt has been taken for development of a protocol for efficient hairy root proliferation system. The Agrobacterium rhizogenes strains (MTCC 532 and MTCC 2364) were used for hairy root induction. For A. rhizogenes infection, both in vitro and in vivo leaves as well as internodes were used as explant. In vitro leaves and internodal explants were obtained by the inoculation of matured nodal segments on the optimum medium [MS + 2.0 mg/L N6-Benzylaminopurine (BAP)] with c.a. 91.6% shoot regeneration and an average of 8.1 shoots per explants. In vitro leaf showed best hairy root induction followed by in vitro internode on ½ MS medium augmented with acetosyringone. Highest transformation efficiency was achieved using MTCC 2364 strain, while no transformation was observed in MTCC 532 strain. Different factors affecting transformation including co-cultivation period, infection time and optical density (O.D.) value were standardized. The highest efficacy, 88.8% hairy root induction was observed in in vitro leaves infected by MTCC 2364 for 60 minutes infection time with an O.D. value of 0.29 maintained over a 44-48 hours of co-cultivation period. The prescribed protocol may be used as a reference for development of industrial scale hairy root production for bioactive compound located in root of V. negundo.
Anahtar Kelimeler Acetosyringone, A. rhizogenes, Co-cultivation period, Hairy root culture, Vitex negundo
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Agrobacterium rhizogenes mediated hairy root induction in endangered Berberis aristata DC. Springer plus, 4(1), 443. https://doi.org/10.1186/s40064-015-1222-1 Chawla, A.S., Sharma, A.K., Handa, S.S., & Dhar, K.L. (1992). Chemical investigation and anti-inflammatory activity of Vitex negundo seeds. Journal of Natural Products, 55(2), 163-167. https://doi.org/10.1021/np50080a002 Dewade, D.R., Christina, A.J.M., Chidambaranathan, N., Bhajipale, N.S., & Tekade, N.P. (2010). Antitumor activity of Vitex negundo Linn. against Dalton’s ascitic lymphoma. International Journal of Pharm Tech Research, 2(2), 1101-1104. Gade, S., Jadhav, R., Vikhe, S. (2023). Phytochemical studies and antiurolithiatic activity of Vitex negundo Linn root extracts. World Journal of Pharmaceutical Science and Research, 2(5), 104-114. Gelvin, G.B. (2000). Agrobacterium and plant genes involved in T-DNA transfer and integration. Annual Review of Plant Physiology and Plant Molecular Biology, 51, 223-256. https://doi.org/10.1146/annurev.arplant.51.1.223 Ghani, A. (1998). Medicinal plants of Bangladesh. Asiatic Society of Bangladesh, Dhaka, Bangladesh, 319‐320. Giri, A., Giri, C.C., Dhingra, V., & Narasu, M.L. (2001). Enhanced podophyllotoxin production from Agrobacterium rhizogenes transformed cultures of Podophyllum hexandrum. Natural Product Letters, 15(4), 229-35. http://dx.doi.org/10.1080/10575630108041286 Godwin, I., Todd, G., Ford-Lloy, B., & Newbury, H.J. (1991). The effects of acetosyringone and pH on Agrobacterium-mediated transformation vary according to plant species. Plant Cell Reports, 9, 671-675. http://dx.doi.org/10.1007/BF00235354 Jesudass., Rajangam, U., Muthukrishnan, A., Andy, G., Mona, S.A., Norah, S.A., & Abubaker, M.A.M. (2020). Effect of different Agrobacterium rhizogenes strains for in-vitro hairy root induction, totalphenolic, flavonoids contents, antibacterial and antioxidant activity of (Cucumis anguria L.). Saudi Journal of Biological Sciences, 27(11), 2972–2979. http://dx.doi.org/10.1016/j.sjbs.2020.08.050 Ji, H., Yang, B., Jing, Y., Luo, Y., Li, B., & Yan, Y. (2023). Trehalose and brassinolide enhance the signature ingredient accumulation and anti-oxidant activity in the hairy root cultures of Polygala tenuifolia Wild. Industrial Crops and Products, 196, 116521. https://doi.org/10.1016/j.indcrop.2023.116521 Kamal, N., Asni, N.S.M., Rozlan, I.N.A., Azmi, M.A.H.M., Mazlan, N.W., Mediani, A., Baharum, S.N., Latip, J., Assaw, S., & Edrada-Ebel, R.A. (2022). Traditional Medicinal Uses, Phytochemistry, Biological Properties, and Health Applications of Vitex sp. Plants, 11(15), 1944. http://dx.doi.org/10.3390/plants11151944 Kumar, A., Kumari, A., Demiwal, P., Roy, P., & Sircar, D. (2023). Enhanced production of bioactive plumbagin in hairy root cultures and adventitious root cultures of Plumbago zylanica L. by a novel apocarotenoid elicitor, α-ionone. Industrial Crops and Products, 203, 117140. https://doi.org/10.1016/j.indcrop.2023.117140 Kumar, V., Sharma, A., Prasad, B.C.N., Gururaj, H.B., & Ravishankar, G.A. (2006). Agrobacterium rhizogenes mediated genetic transformation resulting in hairy root formation is enhanced by ultrasonication and acetosyringone treatment. Electronic Journal of Biotechology, 9(4), 349–357. Ladda, P.L., & Magdum, C.S. (2012). Vitex negundo Linn.: ethnobotany, phytochemistry and pharmacology- a review. International Journal of Advances in Pharmacy Biology and Chemistry, 1(1), 111-120. Mahendran, G., Verma, N., Singh, M., Shanker, K., Banerjee, S., Kumar, B., Rahman, L. (2022). Elicitation enhances swerchirin and 1, 2, 5, 6-tetrahydroxyxanthone production in hairy root cultures of Swertia chirayita (Roxb.) H. Karst. Industrial Crops and Products, 177, 114488. http://dx.doi.org/10.1016/j.indcrop.2021.114488 Majumdar, S., Garai, S., Jha, S. (2011). Genetic transformation of Bacopa monnieri by wild type strains of Agrobacterium rhizogenes stimulates production of Bacopa saponins in transformed calli and plants. Plant Cell Reports, 30, 941 954. http://dx.doi.org/10.1007/s00299-011-1035-9 Moola, A.K., Thiruppathi, S.K., Ranjitha Kumari, B.D. (2021). Enhancement of Celastrol compound by silver nanoparticles and acetosyringone in Celastrus paniculatus Wild. through adventitious and hairy root culture. Journal of plant biochemistry and biotechnology, 31(4). http://dx.doi.org/10.1007/s13562-021-00676-y Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473 497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x Muthiah, J.V.L., Lakkakula, S., Rajaiah, J., Jayabalan, S., & Manikandan, R. (2016). Analysis of propagation of Bacopa monnieri (L.) from hairy roots, elicitation and Bacoside A contents of Ri transformed plants. World Journal of Microbiology and Biotechnology, 32,131. https://doi.org/10.1007/s11274-016-2083-7 Nadkarni, K.M. (2002). Indian Materia Medica, Bombay Popular Prakashan, (vol 1), 1278-1280. Orlikowska, T.K., Cranston, H.J., Dyer, W.E. (1995). Factors influencing Agrobacterium tumefaciens mediated transformation and regeneration of the safflower cultivar ‘Centennial’. Plant Cell Tissue and Organ Culture, 40, 85-91. Rana, S., & Rana, K.K. (2014). Review on medicinal usefulness of Vitex negundo Linn. Open Access Library Journal, 1(3), 1-13. http://dx.doi.org/10.4236/oalib.1100508 Sahu, L., Jena S., Swain S.S., Sahoo, S., Chand, P.K. (2013). Agrobacterium rhizogenes-mediated transformation of a multi-medicinal herb, Boerhaavia diffusa L.: optimization of the process and anti-microbial activity against bacterial pathogens causing urinary tract infections. Frontiers in Life Science, 7(3 4), 197 209. https://doi.org/10.1080/21553769.2013.879266 Sajjalaguddam, R., & Paladugu, A. (2016). Influence of Agrobacterium rhizogenes strains and elicitation on hairy root induction and Glycyrrhizin production from Abrus precatorius. Journal of Pharma ceutical Sciences and Research, 8(12), 1353-1357. Shahabzadeh, Z., Heidari, B., Hafez, R.F. (2014). Induction of transgenic hairy roots in Trigonella foenum-graceum co-cultivated with Agrobacterium rhizogenes harboring a GFP gene. Journal of Crop Science and Biotechnology, 16, 263 268. https://doi.org/10.1007/s12892-013-0082-x Singh, K.S., Frdrik, P.G., Ramamurthy, M. (2016). Phytochemical and antibacterial activity of Vitex negundo, Leucasaspera and Abutilon indicum against wound pathogens. International Journal of Current Research in Biology and Medicines, 1, 60-90. Srinivasan, R., Kamalanathan, D., Boobalan, S., Saranyaa, V., Mouliganesh, S., & Seenivasagan, R. (2023). Agrobacterium rhizogenes influences aervine enhancement in hairy root culture of Aerva javanica (Burm.f.) Juss. ex Schult and in silico assessment of human breast cancer activity. Journal of Applied Biology and Biotechnology, 11(4), 148-158. http://dx.doi.org/10.7324/JABB.2023.23350 Swain, S.S., Sahu, L., Barik, D.P., & Chand, P.K. (2010). Agrobacterium × plant factors influencing transformation of ‘Joseph’s coat’ (Amaranthus tricolor L.). Scientia Horticulturae, 125(3), 461-468. .http://dx.doi.org/10.1016/j.scienta.2010.04.034 Usha, P.K., Benjamin, S., Mohanan, K.V., & Raghu, A.V. (2007). An efficient micropropagation system for Vitex negundo L., an important woody aromatic medicinal plant, through shoot tip culture. Research Journal of Botany, 2, 102 107. http://dx.doi.org/10.3923/rjb.2007.102.107 Yunos, N.M., Kean, O.B., Abas, R. (2005). Cytotoxicity evaluations on Vitex negundo anti-inflammatory extracts. Malaysian Journal of Science, 24(1), 213-217. Toplam 34 adet kaynakça vardır.
Ayrıntılar Birincil Dil İngilizce Konular Bitki Biyoteknolojisi Bölüm Makaleler Yazarlar Bhaswatımayee Mahakur RAVENSHAW UNIVERSITY 0000-0002-6404-4593 India
Arpıta Moharana NATIONAL RICE RESEARCH INSTITUTE, CUTTACK, ODISHA 0000-0001-5050-3288 India
Sanjay Kumar Madkamı RAVENSHAW UNIVERSITY 0009-0001-7688-2893 India
Soumendra Kumar Naık RAVENSHAW UNIVERSITY 0000-0002-3479-6911 India
Durga Prasad Barık RAVENSHAW UNIVERSITY 0000-0002-0319-968X India
Erken Görünüm Tarihi 22 Nisan 2024 Yayımlanma Tarihi 3 Haziran 2024 Gönderilme Tarihi 29 Eylül 2023 Yayımlandığı Sayı Yıl 2024 Cilt: 11 Sayı: 2
Kaynak Göster APA Mahakur, B., Moharana, A., Madkamı, S. K., Naık, S. K., vd. (2024). Optimization of factors affecting Agrobacterium-mediated hairy root induction in Vitex negundo L. (Lamiaceae). International Journal of Secondary Metabolite, 11(2), 244-254. https://doi.org/10.21448/ijsm.1368677
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