Multi-Etiological Abortion due to Campylobacter spp. and Chlamydia abortus in a Sheep

Yıl 2024, Cilt: 17 Sayı: 2, 169 – 175, 30.06.2024

İbrahim Deniz , Ertan Oruç

https://doi.org/10.30607/kvj.1457393

Öz

Bu vakada, Konya Veteriner Kontrol Enstitüsü’ne (KVKE) 2020 yılında, Aksaray ilinde faaliyet gösteren bir koyun işletmesinden gönderilen aborte koyun fetusunda Campylobacter spp. ve Chlamydia abortus (C. abortus) etkenlerine bağlı multi-etiyolojik abort belirlendi. Campylobacter spp. varlığı bakteriyel izolasyon yöntemi ile belirlendi. C. abortus ise qPZR ve immunohistokimya (İHK) yöntemleri ile tanımlandı. Bu çalışmayla, koyun abortlarıyla mücadelede birden çok etkenin karıştığı multi-etiyolojik abortların dikkate alınması, abort etkenlerini tespit etmek için teşhiste eş zamanlı multidisipliner bir yaklaşımın kullanılmasının uygun olacağı ve abortlarla daha etkin bir mücadeleye katkıda bulunulabileceğini belirtmek amaçlanmıştır.

Anahtar Kelimeler

Campylobacter spp., Chlamydia abortus, İmmunohistokimya, Multi-etiyolojik abort, Pzr

Kaynakça

  • Akashi, H., Onuma, S., Nagano, H., Ohta, M., & Fukutomi, T. (1999). Detection and differentiation of Aino and Akabane Simbu serogroup bunyaviruses by nested polymerase chain reaction. Archives of virology, 144(11), 2101–2109. https://doi.org/10.1007/s007050050625
  • Alton, G. G., Jones, L. M., Angus, R. D., & Verger, J. M. (1988). Techniques for the brucellosis laboratory. Institut National de la recherche Agronomique (INRA).
  • Ay, SS. (2017). Küçük ruminatlarda abortus sorunu ve reprodüktif aşılama programları. Turkiye Klinikleri J Vet Sci Obstet Gynecol-Special Topics, 3(2), 129-36.
  • Aydin, F., Murat, A. B. A. Y., Atasever, A., Bayram, L. C., Karakaya, E., Seçil, A. B. A. Y., … & Diker, K. S. (2020). Ovine Abortion Associated with Campylobacter fetus subsp. fetus ST2 in Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 26(4).
  • Barratt, J., Al Qassab, S., Reichel, M. P., & Ellis, J. T. (2008). The development and evaluation of a nested PCR assay for detection of Neospora caninum and Hammondia heydorni in feral mouse tissues. Molecular and cellular probes, 22(4), 228–233. https://doi.org/10.1016/j.mcp.2008.03.001
  • Buyuk, F., Celebi, O., Sahin, M., Unver, A., & Tazegul, E. (2011). İki farklı koyun ve keçi sürüsünde Brucella ve Campylobacter ortak enfeksiyonu. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 17(1). http//doi.org/10.9775/kvfd.2010.3134
  • Costa, J. M., Pautas, C., Ernault, P., Foulet, F., Cordonnier, C., & Bretagne, S. (2000). Real-time PCR for diagnosis and follow-up of Toxoplasma reactivation after allogeneic stem cell transplantation using fluorescence resonance energy transfer hybridization probes. Journal of clinical microbiology, 38(8), 2929–2932. https://doi.org/10.1128/JCM.38.8.2929-2932.2000
  • Couacy-Hymann, E., Roger, F., Hurard, C., Guillou, J. P., Libeau, G., & Diallo, A. (2002). Rapid and sensitive detection of peste des petits ruminants virus by a polymerase chain reaction assay. Journal of virological methods, 100(1-2), 17–25. https://doi.org/10.1016/s0166-0934(01)00386-x
  • De Angelis, M. E., Martino, C., Chiaverini, A., Di Pancrazio, C., Di Marzio, V., Bosica, S., … & Pomilio, F. (2022). Co- Infection of L. monocytogenes and Toxoplasma gondii in a Sheep Flock Causing Abortion and Lamb Deaths. Microorganisms, 10(8), 1647. http//doi.org/10.3390/microorganisms.10081647
  • Deniz, İ., & Oruç, E. (2023). Multietiological abortion induced by Brucella melitensis and Chlamydophila abortus in a sheep fetus. Etlik Veteriner Mikrobiyoloji Dergisi, 34(2), 205-210. http//doi.org/10.35864/evmd.1289382
  • Erdem, H., & Sarıbay, M. K. (2019). Gebelik ve tanı yöntemleri ‘‘Çiftlik Hayvanlarında Doğum ve Jinekoloji’’, Editörler: Mustafa Kaymaz, Murat Fındık, Ali Rişvanlı, Afşin Köker, 3.
  • Gülaydın, Ö., Öztürk, C., Ekin, İ. H., İlhan, Z., & İlhan, F. (2023). Investigation of selected bacterial agents causing sheep abortion in the Van Province by RT-PCR and histopathological methods. Acta Veterinaria Brno, 92(1),69-77. http//doi.org/10.2754/a.vb.2023.92010069
  • Hofmann, M., Griot, C., Chaignat, V., Perler, L., & Thür, B. (2008). Blauzungenkrankheit erreicht die Schweiz [Bluetongue disease reaches Switzerland]. Schweizer Archiv fur Tierheilkunde, 150(2), 49–56. https://doi.org/10.1024/0036- 7281.150.2.49
  • Jinneman, K. C., Hunt, J. M., Eklund, C. A., Wernberg, J. S., Sado, P. N., Johnson, J. M., Richter, R. S., Torres, S. T., Ayotte, E., Eliasberg, S. J., Istafanos, P., Bass, D., Kexel-Calabresa, N., Lin, W., & Barton, C. N. (2003). Evaluation and interlaboratory validation of a selective agar for phosphatidylinositol-specific phospholipase C activity using a chromogenic substrate to detect Listeria monocytogenes from foods. Journal of food protection, 66(3), 441–445. https://doi.org/10.4315/0362-028x-66.3.441
  • Karakurt, E., Nuhoğlu, H., Dağ, S., Sağlam, A. G., Beytut, E., Şahin, M., … & Çelebi, Ö. (2020). Investigation of Campylobacteriosis in Abort Cases in Kars Province by Pathological, Immunohistochemical, PCR and Microbiological Methods. Veterinary Journal of Mehmet Akif Ersoy University, 5(2), 69-74.
  • Lastovica, A. J., & Allos, B. M. (2008). Clinical significance of Campylobacter and related species other than Campylobacter jejuni and Campylobacter coli. Campylobacter, 123-149. http//doi.org/10.1128/9781555815554.ch7
  • Livingstone, M., Wheelhouse, N., Ensor, H., Rocchi, M., Maley, S., Aitchison, K., … & Longbottom, D. (2017). Pathogenic outcome following experimental infection of sheep with Chlamydia abortus variant strains LLG and POS. PLoS One, 12(5), http//doi.org/e0177653.10.1371/journal.pone.0177653
  • Malal, M. E., & Turkyilmaz, S. (2021). Identification and genotyping of Chlamydia abortus with MLVA from ruminant abortions in the Marmara region of Turkey. The Thai Journal of Veterinary Medicine, 51(1), 169-175. http//doi.org/10.14456/tjvm.2021.22
  • Martin, K. W., Mattick, K. L., Harrison, M., & Humphrey, T. J. (2002). Evaluation of selective media for Campylobacter isolation when cycloheximide is replaced with amphotericin B. Letters in applied microbiology, 34(2), 124–129. https://doi.org/10.1046/j.1472-765x.2002.01058.x
  • Otter, A., Twomey, D. F., Rowe, N. S., Tipp, J. W., McElligott, W. S., Griffiths, P. C., & O'Neill, P. (2003). Suspected chlamydial keratoconjunctivitis in British cattle.
  • Pantchev, A., Sting, R., Bauerfeind, R., Tyczka, J., & Sachse, K. (2009). New real-time PCR tests for species-specific detection of Chlamydophila psittaci and Chlamydophila abortus from tissue samples. The Veterinary Journal, 181(2), 145-150. http//doi.org/10.1016/j.tvjl.2008.02.025
  • Ramo, M. D. L. A., Benito, A. A., Quílez, J., Monteagudo, L. V., Baselga, C., & Tejedor, M. T. (2022). Coxiella burnetii and co-infections with other major pathogens causing abortion in small ruminant flocks in the Iberian Peninsula. Animals, 12(24), 3454. http//doi.org/10.3390/ani12243454
  • Sakmanoğlu, A., Uslu, A., Sayın, Z., Gölen, G. S., İlban, A., Padron-Perez, B., Karyeyen, Y., Gök, A., Tekindal, M. A., & Erganis, O. (2021). A one-year descriptive epidemiology of zoonotic abortifacient pathogen bacteria in farm animals in Turkey. Comparative immunology, microbiology and infectious diseases, 77, 101665. https://doi.org/10.1016/j.cimid.2021.101665
  • Şevik, M., Gülcü, Y., & Doğan, M. (2017a). Co-infection with border disease virus and Brucella melitensis in an aborted sheep foetus. Etlik Veteriner Mikrobiyoloji Dergisi, 28(2), 65-68. . http//doi.org/10.35864/evmd.516685 Sheep Aborted Foetus with Peste des Petits Ruminants Virus and Brucella melitensis. Etlik Veteriner Mikrobiyoloji Dergisi, 28(2), 61-64. http//doi.org/10.35864/evmd.516682
  • Tuzcu, M., Oruc, E., Tuzcu, N., Yoldas, A., & Yiğin, A. (2010). Diagnosis of Campylobacteriosis in the aborted bovine fetuses by pathological, immunohistochemical, microbiological and real time PCR. Kafkas Univ Vet Fak Derg, 16, 509- 514. Vilbek, S., & Paton, D. J. (2000). A RT-PCR assay for the rapid recognition of border disease virus. Veterinary research, 31(4), 437–445. https://doi.org/10.1051/vetres:2000130 World Organisation For Animal Health. (2018). Manual of Diagnostic Tests and Vaccines for Terrestrial Animals Chapter 3.8.5. Enzootic Abortion Of Ewes (Ovıne Chlamydiosis). [Access Date: 17.05.2024]. World Organisation For Animal Health. (2022). Manual of Diagnostic Tests and Vaccines for Terrestrial Animals Chapter 3.10.7. Salmonellosis (version adopted in May 2022). [Access Date: 17.05.2024].

Multi-Etiological Abortion due to Campylobacter spp. and Chlamydia abortus in a Sheep

Yıl 2024, Cilt: 17 Sayı: 2, 169 – 175, 30.06.2024

İbrahim Deniz , Ertan Oruç

https://doi.org/10.30607/kvj.1457393

Öz

In this case, multi-etiologic abortion due to Campylobacter spp. and Chlamydia abortus (C. abortus) was reported from an aborted sheep fetus sent to Konya Veterinary Control Institute (KVKE) from a sheep farm operating in Aksaray province in 2020. The presence of Campylobacter spp. was determined by the bacterial isolation method. C. abortus was identified by qPCR and immunohistochemistry (IHC) methods. In this study, it was aimed to indicate that multi-etiological abortions involving multiple factors should be taken into consideration in the fight against sheep abortions, that it would be appropriate to use a simultaneous multidisciplinary approach in diagnosis to identify abortion factors, and that it could contribute to a more effective fight against abortions.

Anahtar Kelimeler

Campylobacter spp., Chlamydia abortus, Immunohistochemistry, Multi-etiological abortion, Pcr

Etik Beyan

“This study is not subject to the permission of HADYEK in accordance with the “Regulation on Working Procedures and Principles of Animal Experiments Ethics Committees” 8 (k). The data, information and documents presented in this article were obtained within the framework of academic and ethical rules.” (Ref No: 701825, Date: 02/2024).

Teşekkür

The authors thank the Ministry of Agriculture and Forestry, the General Directorate of Food and Control, and the Konya Veterinary Control Institutes for cooperating in this study.

Kaynakça

  • Akashi, H., Onuma, S., Nagano, H., Ohta, M., & Fukutomi, T. (1999). Detection and differentiation of Aino and Akabane Simbu serogroup bunyaviruses by nested polymerase chain reaction. Archives of virology, 144(11), 2101–2109. https://doi.org/10.1007/s007050050625
  • Alton, G. G., Jones, L. M., Angus, R. D., & Verger, J. M. (1988). Techniques for the brucellosis laboratory. Institut National de la recherche Agronomique (INRA).
  • Ay, SS. (2017). Küçük ruminatlarda abortus sorunu ve reprodüktif aşılama programları. Turkiye Klinikleri J Vet Sci Obstet Gynecol-Special Topics, 3(2), 129-36.
  • Aydin, F., Murat, A. B. A. Y., Atasever, A., Bayram, L. C., Karakaya, E., Seçil, A. B. A. Y., … & Diker, K. S. (2020). Ovine Abortion Associated with Campylobacter fetus subsp. fetus ST2 in Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 26(4).
  • Barratt, J., Al Qassab, S., Reichel, M. P., & Ellis, J. T. (2008). The development and evaluation of a nested PCR assay for detection of Neospora caninum and Hammondia heydorni in feral mouse tissues. Molecular and cellular probes, 22(4), 228–233. https://doi.org/10.1016/j.mcp.2008.03.001
  • Buyuk, F., Celebi, O., Sahin, M., Unver, A., & Tazegul, E. (2011). İki farklı koyun ve keçi sürüsünde Brucella ve Campylobacter ortak enfeksiyonu. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 17(1). http//doi.org/10.9775/kvfd.2010.3134
  • Costa, J. M., Pautas, C., Ernault, P., Foulet, F., Cordonnier, C., & Bretagne, S. (2000). Real-time PCR for diagnosis and follow-up of Toxoplasma reactivation after allogeneic stem cell transplantation using fluorescence resonance energy transfer hybridization probes. Journal of clinical microbiology, 38(8), 2929–2932. https://doi.org/10.1128/JCM.38.8.2929-2932.2000
  • Couacy-Hymann, E., Roger, F., Hurard, C., Guillou, J. P., Libeau, G., & Diallo, A. (2002). Rapid and sensitive detection of peste des petits ruminants virus by a polymerase chain reaction assay. Journal of virological methods, 100(1-2), 17–25. https://doi.org/10.1016/s0166-0934(01)00386-x
  • De Angelis, M. E., Martino, C., Chiaverini, A., Di Pancrazio, C., Di Marzio, V., Bosica, S., … & Pomilio, F. (2022). Co- Infection of L. monocytogenes and Toxoplasma gondii in a Sheep Flock Causing Abortion and Lamb Deaths. Microorganisms, 10(8), 1647. http//doi.org/10.3390/microorganisms.10081647
  • Deniz, İ., & Oruç, E. (2023). Multietiological abortion induced by Brucella melitensis and Chlamydophila abortus in a sheep fetus. Etlik Veteriner Mikrobiyoloji Dergisi, 34(2), 205-210. http//doi.org/10.35864/evmd.1289382
  • Erdem, H., & Sarıbay, M. K. (2019). Gebelik ve tanı yöntemleri ‘‘Çiftlik Hayvanlarında Doğum ve Jinekoloji’’, Editörler: Mustafa Kaymaz, Murat Fındık, Ali Rişvanlı, Afşin Köker, 3.
  • Gülaydın, Ö., Öztürk, C., Ekin, İ. H., İlhan, Z., & İlhan, F. (2023). Investigation of selected bacterial agents causing sheep abortion in the Van Province by RT-PCR and histopathological methods. Acta Veterinaria Brno, 92(1),69-77. http//doi.org/10.2754/a.vb.2023.92010069
  • Hofmann, M., Griot, C., Chaignat, V., Perler, L., & Thür, B. (2008). Blauzungenkrankheit erreicht die Schweiz [Bluetongue disease reaches Switzerland]. Schweizer Archiv fur Tierheilkunde, 150(2), 49–56. https://doi.org/10.1024/0036- 7281.150.2.49
  • Jinneman, K. C., Hunt, J. M., Eklund, C. A., Wernberg, J. S., Sado, P. N., Johnson, J. M., Richter, R. S., Torres, S. T., Ayotte, E., Eliasberg, S. J., Istafanos, P., Bass, D., Kexel-Calabresa, N., Lin, W., & Barton, C. N. (2003). Evaluation and interlaboratory validation of a selective agar for phosphatidylinositol-specific phospholipase C activity using a chromogenic substrate to detect Listeria monocytogenes from foods. Journal of food protection, 66(3), 441–445. https://doi.org/10.4315/0362-028x-66.3.441
  • Karakurt, E., Nuhoğlu, H., Dağ, S., Sağlam, A. G., Beytut, E., Şahin, M., … & Çelebi, Ö. (2020). Investigation of Campylobacteriosis in Abort Cases in Kars Province by Pathological, Immunohistochemical, PCR and Microbiological Methods. Veterinary Journal of Mehmet Akif Ersoy University, 5(2), 69-74.
  • Lastovica, A. J., & Allos, B. M. (2008). Clinical significance of Campylobacter and related species other than Campylobacter jejuni and Campylobacter coli. Campylobacter, 123-149. http//doi.org/10.1128/9781555815554.ch7
  • Livingstone, M., Wheelhouse, N., Ensor, H., Rocchi, M., Maley, S., Aitchison, K., … & Longbottom, D. (2017). Pathogenic outcome following experimental infection of sheep with Chlamydia abortus variant strains LLG and POS. PLoS One, 12(5), http//doi.org/e0177653.10.1371/journal.pone.0177653
  • Malal, M. E., & Turkyilmaz, S. (2021). Identification and genotyping of Chlamydia abortus with MLVA from ruminant abortions in the Marmara region of Turkey. The Thai Journal of Veterinary Medicine, 51(1), 169-175. http//doi.org/10.14456/tjvm.2021.22
  • Martin, K. W., Mattick, K. L., Harrison, M., & Humphrey, T. J. (2002). Evaluation of selective media for Campylobacter isolation when cycloheximide is replaced with amphotericin B. Letters in applied microbiology, 34(2), 124–129. https://doi.org/10.1046/j.1472-765x.2002.01058.x
  • Otter, A., Twomey, D. F., Rowe, N. S., Tipp, J. W., McElligott, W. S., Griffiths, P. C., & O'Neill, P. (2003). Suspected chlamydial keratoconjunctivitis in British cattle.
  • Pantchev, A., Sting, R., Bauerfeind, R., Tyczka, J., & Sachse, K. (2009). New real-time PCR tests for species-specific detection of Chlamydophila psittaci and Chlamydophila abortus from tissue samples. The Veterinary Journal, 181(2), 145-150. http//doi.org/10.1016/j.tvjl.2008.02.025
  • Ramo, M. D. L. A., Benito, A. A., Quílez, J., Monteagudo, L. V., Baselga, C., & Tejedor, M. T. (2022). Coxiella burnetii and co-infections with other major pathogens causing abortion in small ruminant flocks in the Iberian Peninsula. Animals, 12(24), 3454. http//doi.org/10.3390/ani12243454
  • Sakmanoğlu, A., Uslu, A., Sayın, Z., Gölen, G. S., İlban, A., Padron-Perez, B., Karyeyen, Y., Gök, A., Tekindal, M. A., & Erganis, O. (2021). A one-year descriptive epidemiology of zoonotic abortifacient pathogen bacteria in farm animals in Turkey. Comparative immunology, microbiology and infectious diseases, 77, 101665. https://doi.org/10.1016/j.cimid.2021.101665
  • Şevik, M., Gülcü, Y., & Doğan, M. (2017a). Co-infection with border disease virus and Brucella melitensis in an aborted sheep foetus. Etlik Veteriner Mikrobiyoloji Dergisi, 28(2), 65-68. . http//doi.org/10.35864/evmd.516685 Sheep Aborted Foetus with Peste des Petits Ruminants Virus and Brucella melitensis. Etlik Veteriner Mikrobiyoloji Dergisi, 28(2), 61-64. http//doi.org/10.35864/evmd.516682
  • Tuzcu, M., Oruc, E., Tuzcu, N., Yoldas, A., & Yiğin, A. (2010). Diagnosis of Campylobacteriosis in the aborted bovine fetuses by pathological, immunohistochemical, microbiological and real time PCR. Kafkas Univ Vet Fak Derg, 16, 509- 514. Vilbek, S., & Paton, D. J. (2000). A RT-PCR assay for the rapid recognition of border disease virus. Veterinary research, 31(4), 437–445. https://doi.org/10.1051/vetres:2000130 World Organisation For Animal Health. (2018). Manual of Diagnostic Tests and Vaccines for Terrestrial Animals Chapter 3.8.5. Enzootic Abortion Of Ewes (Ovıne Chlamydiosis). [Access Date: 17.05.2024]. World Organisation For Animal Health. (2022). Manual of Diagnostic Tests and Vaccines for Terrestrial Animals Chapter 3.10.7. Salmonellosis (version adopted in May 2022). [Access Date: 17.05.2024].

Toplam 25 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Bakteriyoloji, Veteriner Patoloji, Veteriner Tanı ve Teşhis
BölümOLGU SUNUMU
Yazarlar

İbrahim Deniz Konya Veteriner Kontrol Enstitüsü Müdürlüğü 0000-0001-8526-8396 Türkiye

Ertan Oruç SELÇUK ÜNİVERSİTESİ 0000-0003-4234-8219 Türkiye

Erken Görünüm Tarihi13 Haziran 2024
Yayımlanma Tarihi30 Haziran 2024
Gönderilme Tarihi22 Mart 2024
Kabul Tarihi10 Haziran 2024
Yayımlandığı Sayı Yıl 2024 Cilt: 17 Sayı: 2

Kaynak Göster

APADeniz, İ., & Oruç, E. (2024). Multi-Etiological Abortion due to Campylobacter spp. and Chlamydia abortus in a Sheep. Kocatepe Veterinary Journal, 17(2), 169-175. https://doi.org/10.30607/kvj.1457393
AMADeniz İ, Oruç E. Multi-Etiological Abortion due to Campylobacter spp. and Chlamydia abortus in a Sheep. kvj. Haziran 2024;17(2):169-175. doi:10.30607/kvj.1457393
ChicagoDeniz, İbrahim, ve Ertan Oruç. “Multi-Etiological Abortion Due to Campylobacter Spp. And Chlamydia Abortus in a Sheep”. Kocatepe Veterinary Journal 17, sy. 2 (Haziran 2024): 169-75. https://doi.org/10.30607/kvj.1457393.
EndNoteDeniz İ, Oruç E (01 Haziran 2024) Multi-Etiological Abortion due to Campylobacter spp. and Chlamydia abortus in a Sheep. Kocatepe Veterinary Journal 17 2 169–175.
IEEEİ. Deniz ve E. Oruç, “Multi-Etiological Abortion due to Campylobacter spp. and Chlamydia abortus in a Sheep”, kvj, c. 17, sy. 2, ss. 169–175, 2024, doi: 10.30607/kvj.1457393.
ISNADDeniz, İbrahim – Oruç, Ertan. “Multi-Etiological Abortion Due to Campylobacter Spp. And Chlamydia Abortus in a Sheep”. Kocatepe Veterinary Journal 17/2 (Haziran 2024), 169-175. https://doi.org/10.30607/kvj.1457393.
JAMADeniz İ, Oruç E. Multi-Etiological Abortion due to Campylobacter spp. and Chlamydia abortus in a Sheep. kvj. 2024;17:169–175.
MLADeniz, İbrahim ve Ertan Oruç. “Multi-Etiological Abortion Due to Campylobacter Spp. And Chlamydia Abortus in a Sheep”. Kocatepe Veterinary Journal, c. 17, sy. 2, 2024, ss. 169-75, doi:10.30607/kvj.1457393.
VancouverDeniz İ, Oruç E. Multi-Etiological Abortion due to Campylobacter spp. and Chlamydia abortus in a Sheep. kvj. 2024;17(2):169-75.

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