Bait İşaretleyici Olarak Clenbuterol: Köpeklere Bait’lerin Verilişi

Yıl 1998, Cilt: 9 Sayı: Özel Sayı, 135 – 142, 01.06.1998

Andreas GLEIXNER Orhan AYLAN Heinrich MEYER Ad VOS

Öz

Vahşi hayvanların kuduza karşı ağız yoluyla aşılanmasında kullanılan
baitler işaretleyici olarak tetrasiklini içerir. Bu işaretleyici köpekierin ağız
yoluyla işaretlenmesi için uygun degildir. Bu çalışmada anabalik β2 adrenoceptörü
agonisti olan Clenbuterol’un bait işaretleyici olabileceği test edilmiştir.
Önceki çalışmalarda işaretleyicinin oral uygulamadan sonra kıl uzaması
boyunca kıl teline nüfuz ettiğ;ini göstermiştir. Bu işaretleyicinin kinetigi
laboratuvar denemesi olarak: 0.5 mg Clenbuterol bir bait içine enjekte
edilmiş ve 18 adet köpeğe verilmiştir. Clenbuterol uygulanmasından 28 ve
56 gün sonra Clenbuterol, köpeklerin kıl numunelerinde tesbit edilmiştir.
Buna ilaveten elde edilen sonuçlar numunelerdeki Clenbuterol konsantrasyonu
ile kıl pigmentasyonu yoğunluğu arasındaki ilişkiyi belirgin şekilde
ortaya koymuştur. Bait dağıtım sisteminin kırsal alanlarda seçilen bölgelerde
verilerek fizibilitesinin ölçülmesinde Clenbuterol ve 1.5 x 10⁸ FFU SAD
B 19 aşı virusu ile doldurulmuş aşı container’ı içeren 150 adet bait İstanbul’da
Ferhatpaşa’da kırsal bölgede yol boyunca konulmuştur. Ertesi saball
konulan baitlerin % 93’ü yerinde bulunamamıştır. Bait dağıtımından
önce ve sonra sahipsiz 31 adet köpek yakalanarak kanları alınmış ve bait
dagıtımından sonra kıl numuneleri de alınmıştır. Elde edilen sonuçlar baitlerin
seçilen alanlarda yerleştirilmesinin sahipsiz köpek populasyonuna
ulaşınada etkin bir yol olmadıgı şeklinde sonuçlanan saha çalışmalarını
desteklemektedir. Şöyleki yakalanan sahipsiz köpeklerin sadece %23’de 0.5
IU /ml eşik degerlik kuduz nötralizan antikoru tesbit edilmiştir.

Anahtar Kelimeler

Etlik Veteriner Mikrobiyoloji Dergisi, Köpek, Clenbuterol, Bait

Kaynakça

• Aylan O, Vos A (1997) Efficacy studies with SAD B 19 in Turkish dogs. J . Etlik Vet. Microbiol., 9:93-101
• Coleman PG, Dye C (1996) Immunization coverage required to prevent outbreaks of dog rabies. Vaccine, 14: 185-186 .
• Gleixner A (1996) Pharmakologische Untersuchungen zur Anreicherung von Clenbuterol und steroidalen Anabolilm in pigmentierten Geweben. Herbert Utz Verlag Wissenschaft, München.
• Gleixner A, Sauerwein H, Meyer HHD (1996a) Detection of the anabolic ~2-adrenoceptor agonist Clenbuterol in human scalp hair by HPLC/EIA. Clin . Chem .. 42 : 1869-1871
• Gleixner A, Sauerwein H, Meyer HHD (1996b) Accumulation of the β2-adrenoceptor agonist Clenbuterol in calf hair of different pigmentation. Arch. Lebensmittelhyg., 47:131-135
• Linhart SB (1993) B ait formulation and distribution for oral rabies vaccination of domestic dogs: an overview. Onderstepoort J . Vet. Res .. 60: 479-490.
• Meyer HHD, Rinke LM (1991) The pharmacokinetics and residues of clenbuterol in veal calves. J. Anim. Sc .. 69:4538-4544
• Müller Th, Schlüter H (1998) Oral immunization of red foxes (Vulpes vulpes L.) in Europe – a review. J. Etlik Vet. Microbiol., 9:35-60
• Schuster P, Gülsen N, Neubert A, Vos A (1998) Field trials evaluating bait uptal{e by an urban dog population in Turkey. J. Etlik Vet. Microbiol.. 9:73-81
• V os A, Şanlı S (1998) Evaluation of bait delivery systems for oral vaccination of dogs against rabies in Turkey. J. Etlik Vet. Microbiol.. 9:83-91

The Delivery of Baits to Dogs: Clenbuterol as Baitmarker

Yıl 1998, Cilt: 9 Sayı: Özel Sayı, 135 – 142, 01.06.1998

Andreas GLEIXNER Orhan AYLAN Heinrich MEYER Ad VOS

Öz

Baits used for oral vaccination of wildlife against rabies contain the
marker tetracycline . This marker is not suitable for oral vaccination of dogs. In this study the possibility of the anabolic β2-adrenoceptor agonist
Clenbuterol as bait marker was tested. In previous studies it was shown
that the marker is incorporated into the hair fibre during hair growth after
oral a pplication. The kinetics of this marker was studied in a laboratory experiment: 0.5mg Clenbuterol was injected in baits and offered to 18
dogs . 28 and 56 daysafter application Clenbuterol could be detected in hair
samples of the dogs. Furthermore, the results obtained showeel clearly the
relationship between the concentration of Clenbuterol in the samples and
the intensity of hair pigmentation. To test the feasibility of the bait delivery
system by placing baits at selected sitesin urban areas, 150 baits containing
Clenbu ter ol and a vaccine container filled with 1.5 x 10⁸ FFU SAD B 19
vaccine virus were distributed along roads in the urban neighbourhood
Ferhatpaşa in İstanbul. The next rooming 93% of the baits had disappeared.
Before and after bait distribution 62 and 31 ‘ownerless’ dogs were caught
and recaptured and bled on these occasions, respectively, during the latter
alsa a hair sample was taken. The results supported previous field-studies
that placing baits at selected si tes is not a very efficient way of reaching the
ownerless dog population. Only 23% of the recaptured ownerless dogs had
a rabies neutralizing antibody titre above the threshold of 0 .5 lU /ml.

Kaynakça

• Aylan O, Vos A (1997) Efficacy studies with SAD B 19 in Turkish dogs. J . Etlik Vet. Microbiol., 9:93-101
• Coleman PG, Dye C (1996) Immunization coverage required to prevent outbreaks of dog rabies. Vaccine, 14: 185-186 .
• Gleixner A (1996) Pharmakologische Untersuchungen zur Anreicherung von Clenbuterol und steroidalen Anabolilm in pigmentierten Geweben. Herbert Utz Verlag Wissenschaft, München.
• Gleixner A, Sauerwein H, Meyer HHD (1996a) Detection of the anabolic ~2-adrenoceptor agonist Clenbuterol in human scalp hair by HPLC/EIA. Clin . Chem .. 42 : 1869-1871
• Gleixner A, Sauerwein H, Meyer HHD (1996b) Accumulation of the β2-adrenoceptor agonist Clenbuterol in calf hair of different pigmentation. Arch. Lebensmittelhyg., 47:131-135
• Linhart SB (1993) B ait formulation and distribution for oral rabies vaccination of domestic dogs: an overview. Onderstepoort J . Vet. Res .. 60: 479-490.
• Meyer HHD, Rinke LM (1991) The pharmacokinetics and residues of clenbuterol in veal calves. J. Anim. Sc .. 69:4538-4544
• Müller Th, Schlüter H (1998) Oral immunization of red foxes (Vulpes vulpes L.) in Europe – a review. J. Etlik Vet. Microbiol., 9:35-60
• Schuster P, Gülsen N, Neubert A, Vos A (1998) Field trials evaluating bait uptal{e by an urban dog population in Turkey. J. Etlik Vet. Microbiol.. 9:73-81
• V os A, Şanlı S (1998) Evaluation of bait delivery systems for oral vaccination of dogs against rabies in Turkey. J. Etlik Vet. Microbiol.. 9:83-91

Ayrıntılar

Kaynak Göster

Download or read online: Click here