Yıl 1998, Cilt: 14 Sayı: 2, 121 – 129, 01.06.1998
Öz
Bu çalışmada, köpeklerde taze ve donmuş-çözünmüş spermada anormal spermatozoon oranı ve tiplerinin tespit edilmesinde larldı Hancock solusyonu, Eosin·nigrosin, Hematoksilen·eosin ve Caserett boyaları kuJlanılarak anormal spematozoon oran· lan belirlendi. Ejakülatın geri kalan kısmı Tris-Frukloz sulandırıcısı kullanılarak 0.25 ml’lik payeller içerisinde don· duruldu. Payeller çözdürüldüklen sonra taze spermada anormal spermatozoon oranını tespit etmek amacı ile yapılan tüm muayeneler aynı yöntemler kullanılarak tekrar edildi. Anormal spermaıozoon oranlarının tespiti için kullanılan yöntemler arasında Istatistiki lark gözlenmedi (p>0.05). Sonuç olarak; taze ve donmuş-çôzünmüş spermada anormal spermatozoon oranlannın belirlenebilmesi Için Hancock solusyonu yanında farklı boyama yönıemlerinin de başarıyla kullanılabileceği kanısına varıldı
Anahtar Kelimeler
Köpek, anormal spermatozoon, boyama yöntemleri, taze ve çözünmüş sperma
Effectiveness of the Various Staining Methods to Detect Abnormal Sperm Types and Rates in Fresh and Frozen-thawed Dog Semen
Yıl 1998, Cilt: 14 Sayı: 2, 121 – 129, 01.06.1998
Öz
Ef1ectiveness of the various stalning methods to detect abnormal sperm types and rates in fresh and frozen·lhawed dog semen were studied. Semen samples were colleCled by digital manipulation. Abnormal sperm rates ollresh semen samples were deıermined lollowing Ihe fixaıion in Hanrock solution or in the smears stained by eosinnigrosin, hematoksilen-eosln and Caserette stains. Morphological examinalion ol the semen sanyles were also con· ducted lollowing Ireezing and thawing in Tris-lructose extender in 0.25 ml of French straw. Analyses ol lhe data shawed Ihat there is no slatistic difference (p>O.OS) among the fixation and stain groups to determine abnormal sperm types and rales of fresh or Irozen·thawed dog semen. As a conclusion; in addition lo Haneock solution. dilleren sla· ining methods can be used to delect morphologically abnormal sperm in Iresh and frozen-thawed dog semen
Anahtar Kelimeler
Dog, abnormal spermatozoon, staining method, fresh and thawed semen
Ayrıntılar
| Diğer ID | JA73ET54KC |
|---|---|
| Bölüm | Araştırma |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Haziran 1998 |
| Yayımlandığı Sayı | Yıl 1998 Cilt: 14 Sayı: 2 |
Kaynak Göster
| APA | Ataman, M. B., Yıldız, C., Kaya, A., Aksoy, M., vd. (1998). Effectiveness of the Various Staining Methods to Detect Abnormal Sperm Types and Rates in Fresh and Frozen-thawed Dog Semen. Eurasian Journal of Veterinary Sciences, 14(2), 121-129. | |
| AMA | Ataman MB, Yıldız C, Kaya A, Aksoy M, Lehimcioğlu N. Effectiveness of the Various Staining Methods to Detect Abnormal Sperm Types and Rates in Fresh and Frozen-thawed Dog Semen. Eurasian J Vet Sci. Haziran 1998;14(2):121-129. | |
| Chicago | Ataman, M. Bozkurt, Cengiz Yıldız, Abdullah Kaya, Melih Aksoy, ve Necdet Lehimcioğlu. “Effectiveness of the Various Staining Methods to Detect Abnormal Sperm Types and Rates in Fresh and Frozen-Thawed Dog Semen”. Eurasian Journal of Veterinary Sciences 14, sy. 2 (Haziran 1998): 121-29. | |
| EndNote | Ataman MB, Yıldız C, Kaya A, Aksoy M, Lehimcioğlu N (01 Haziran 1998) Effectiveness of the Various Staining Methods to Detect Abnormal Sperm Types and Rates in Fresh and Frozen-thawed Dog Semen. Eurasian Journal of Veterinary Sciences 14 2 121–129. | |
| IEEE | M. B. Ataman, C. Yıldız, A. Kaya, M. Aksoy, ve N. Lehimcioğlu, “Effectiveness of the Various Staining Methods to Detect Abnormal Sperm Types and Rates in Fresh and Frozen-thawed Dog Semen”, Eurasian J Vet Sci, c. 14, sy. 2, ss. 121–129, 1998. | |
| ISNAD | Ataman, M. Bozkurt vd. “Effectiveness of the Various Staining Methods to Detect Abnormal Sperm Types and Rates in Fresh and Frozen-Thawed Dog Semen”. Eurasian Journal of Veterinary Sciences 14/2 (Haziran 1998), 121-129. | |
| JAMA | Ataman MB, Yıldız C, Kaya A, Aksoy M, Lehimcioğlu N. Effectiveness of the Various Staining Methods to Detect Abnormal Sperm Types and Rates in Fresh and Frozen-thawed Dog Semen. Eurasian J Vet Sci. 1998;14:121–129. | |
| MLA | Ataman, M. Bozkurt vd. “Effectiveness of the Various Staining Methods to Detect Abnormal Sperm Types and Rates in Fresh and Frozen-Thawed Dog Semen”. Eurasian Journal of Veterinary Sciences, c. 14, sy. 2, 1998, ss. 121-9. | |
| Vancouver | Ataman MB, Yıldız C, Kaya A, Aksoy M, Lehimcioğlu N. Effectiveness of the Various Staining Methods to Detect Abnormal Sperm Types and Rates in Fresh and Frozen-thawed Dog Semen. Eurasian J Vet Sci. 1998;14(2):121-9. |