Ordu’nun Mesudiye İlçesi Keçilerinde anti- Neospora caninum Antikorlarının ELISA Testi ile Araştırılması

Yıl 2021, Cilt: 14 Sayı: 1, 1 – 5, 31.03.2021

Deniz Özdamar Bilge Karatepe , Alparslan Yıldırım

https://doi.org/10.30607/kvj.747033 Cited By: 1

Öz

Bu çalışma, Ordu’nun Mesudiye ilçesi keçilerinde Neospora caninum seroprevalansının saptanması amacı ile yapılmıştır. Araştırmada Mesudiye ilçesindeki 5 farklı çalışma merkezine ait (Arpaalan, Birebir, Çiftliksarıca, Yeşilçit ve Musalı) 1 yaş ve üzerinde rastgele seçilen toplam 184 dişi keçinin kan serumlarında N. caninum antikorları ELISA testi ile incelenmiştir. Çalışma ile Ordu’nun Mesudiye ilçesinde incelenen toplam 184 dişi keçinin 16’sı (%8.69) anti-N. caninum antikorları yönünden seropozitif olarak saptanmıştır. Yaş grupları açısından, en fazla seropozitiflik oranı %13.04 ile iki yaşındaki keçilerde belirlenmiş, bir yaşındaki keçilerde ise seropozitiflik saptanamamıştır. Çalışma merkezleri arasında en yüksek seroprevalans %36.6 ile Birebir’de belirlenmiş, Çiftliksarıca (Nisan) ve Yeşilçit’de ise seropozitiflik saptanamamıştır. Ayrıca abort görülmeyen 178 keçinin 16’sı (%8.98) seropozitif bulunurken, abort geçmişi olan 6 keçinin hiçbirinde seropozitiflik tespit edilememiştir. Neospora caninum seroprevalans oranları keçilerin yaş grupları ile abort görülen ve görülmeyen keçiler arasında istatistiksel olarak önemsiz bulunmuş (P>0.05), çalışma merkezleri açısından ise seropozitifliğin önemli olduğu saptanmıştır (P<0.001). Ordu’nun Mesudiye ilçesinde keçilerde Neospora caninum ilk defa bu çalışma ile belirlenmiş ve %8.69 oranında seropozitiflik saptanmıştır.

Anahtar Kelimeler

Neospora caninum, neosporosis, keçi, Ordu–Mesudiye, seroprevalans, ELISA

Destekleyen Kurum

Niğde Ömer Halisdemir Üniversitesi Bilimsel Araştırma Projeleri Birimi

Proje Numarası

FEB 2013/32-YÜLTEP

Teşekkür

Niğde Ömer Halisdemir Üniversitesi Bilimsel Araştırma Projeleri Birimi tarafından FEB 2013/32-YÜLTEP kodu ile desteklenmiştir. İlk isim yazarın Yüksek Lisans Tezi’nden özetlenmiştir.

Kaynakça

• Al-Majali AM, Jawasreh KI, Talafha HA, Talafha AQ. Neosporosis in Sheep and Different Breeds of Goats from Southern Jordan: Prevalence and Risk Factors Analysis. Am J Anim Vet Sci. 2008; 3: 47-52.
• Anastasia D, Elias P, Nikolaos P, Charilaos K, Nektarios G. Toxoplasma gondii and Neospora caninum seroprevalence in dairy sheep and goats mixed stock farming. Vet Parasitol. 2013; 198: 387-390.
• Bartova E, Sedlak K. Toxoplasma gondii and Neospora caninum antibodies in goats in the Czech Republic. Vet Med-Czech. 2012; 57: 111-114.
• Björkman C, Uggla A. Serological diagnosis of Neospora caninum infection. Int J Parasitol. 1999; 29: 1497-1507.
• Bowman DD, Lynn RC, Eberhard ML. Georgis Parasitology for Veterinarians. Elsevier Science, USA, 2003.
• Cayvaz M, Karatepe M. Niğde Yöresi Keçilerinde Neospora caninum’un Seroprevalansı. Kafkas Univ Vet Fak Derg. 2011; 17: 935-939.
• Cobadiova, A., Reiterova, K., Derdakova, M., Spilovska, S., Turcekova, L., Hviscova, I. Hisira, V. Toxoplasma gondii, Neospora caninum and tick-transmitted bacterium Anaplasma phagocytophilum infections in one selected goat farm in Slovakia. Acta Parasitol 2013; 58: 541-546.
• Czopowicz M, Kaba J, Szalu´s-Jordanow O, Nowicki M, Witkowski L, Frymus T. Seroprevalence of Toxoplasma gondii and Neospora caninum infections in goats in Poland. Vet Parasitol. 2011; 178: 339–341.
• Dubey JP. Review of Neospora caninum and neosporosis in animals. Korean J Parasitol. 2003; 41: 1-16.
• Dubey JP, Carpenter JL, Speer CA, Topper MJ, Uggla A. Newly recognized fatal protozoon disease of dogs. J Am Vet Med Assoc. 1988; 192: 1269-1285.
• Dubey JP, Schares G, Ortega-Mora LM. Epidemiology and Control of Neosporosis and Neospora caninum. Clin Microbiol Rev. 2007; 20: 323-367.
• Faria EB, Gennari SM, Pena HFJ, Athayde ACR, Silva MLCR, Azevedo SS. Prevalence of anti- Toxoplasma gondii and anti-Neospora caninum antibodies in goats slaughtered in the public slaughterhouse of Patos city, Paraı’ba State, Northeast region of Brazil. Vet Parasitol. 2007; 149:126-129.
• Figliuolo LPC, Rodrigues AAR, Viana RB, Aguiar DM, Kasai N, Gennari SM. Prevalence of anti-Toxoplasma gondii and anti-Neospora caninum antibodies in goat from São Paulo State, Brazil. Small Ruminant Res. 2004; 55: 29-32.
• Iovu A, Györke A, Mircean V, Gavrea R, Cozma V. Seroprevalence of Toxoplasma gondii and Neospora caninum in dairy goats from Romania. Vet Parasitol 2012; 186: 470-474.
• McAllister MM, Dubey JP, Lindsay DS, Jolley WR, Wills RA, McGuire AM. Dogs are definitive hosts of Neospora caninum. Int J Parasitol. 1998; 28: 1473-1478.
• Moreno B, Collantes-Fernandez E, Villa A, Navarro A, Regidor-Cerrillo J, Ortega-Mora LM. Occurrence of Neospora caninum and Toxoplasma gondii infections in ovine and caprine abortions. Vet Parasitol. 2012; 187: 312-318.
• Naguleswaran A, Hemphill A, Rajapakse RPVJ. Sager H. Elaboration of a crude antigen ELISA for serodiagnosis of caprine neosporosis: validation of the test by detection of Neospora caninum-specific antibodies in goats from Sri Lanka. Vet Parasitol. 2004; 126: 257-262.
• Sevgili M, Çimtay İ, Keskin O. Şanlıurfa Yöresindeki Keçilerde Neospora caninum Enfeksiyonunun Seroprevalansı. Türkiye Parazitol Derg. 2003; 27: 249-251.
• Şuteu O, Paştiu A, Györke A, Avram E, Cozma V. Molecular detection of Neospora caninum in slaughtered goat kids from Romania. Sci Parasitol. 2013; 14: 43-46.
• Tembue AASM, Ramos RAN, Sousa TR, Albuquerque AR, Costa AJ, Meunier IMJ, Faustino MAG, Alves LC. Serological survey of Neospora caninum in small ruminants from Pernambuco State, Brazil. Rev Bras Parasitol Vet. 2011; 20: 246-248.
• Topazio JP, Weber A, Camillo G, Vogel FF, Machado G, Ribeiro A, Moura AB, Lopes LS, Tonin AA, Solda NM, Braunig P, Silva AS. Seroprevalence and risk factors for Neospora caninum in goats in Santa Catarina state, Brazil. Rev Bras Parasitol Vet. 2014; 23: 360-366.
• Unzaga JM, More G, Bacigalupe D, Rambeaud M, Pardini L, Dellarupe A, Felice LD, Gos ML, Venturini MC. Toxoplasma gondii and Neospora caninum infections in goat abortions from Argentina. Parasitol Int. 2014; 63: 865-867.
• Utuk AE, Simsek S, Piskin FC, Balkaya I. Detection of Neospora caninum IgG Antibodies in Goats in Elazig, Erzurum and Kırsehir Provinces of Turkey. Isr J Vet Med. 2011; 66: 157-160.
• Uzeda RS, Pinheiro AM, Fernandez SY, Ayres MCC, Gondim LFP, Almeida MAO. Seroprevalence of Neospora caninum in dairy goats from Bahia, Brazil. Small Ruminant Res. 2007; 70: 257–259.

Investigation of anti-Neospora caninum Antibodies in Goats in Mesudiye District of Ordu using ELISA

Yıl 2021, Cilt: 14 Sayı: 1, 1 – 5, 31.03.2021

Deniz Özdamar Bilge Karatepe , Alparslan Yıldırım

https://doi.org/10.30607/kvj.747033 Cited By: 1

Öz

This research was carried out to investigate the seroprevalence of Neospora caninum in goats of Mesudiye district in Ordu province. A total of 184 female goats, which were a year old or more, were randomly selected from five different study sites (Arpaalan, Birebir, Çiftliksarıca, Yeşilçit and Musalı) and the serum samples of the goats were analyzed for anti-N.caninum antibodies by applying ELISA. 16 out of 184 sera (8.69%) were determined as seropositive for anti-N.caninum antibodies. In terms of age groups, the seropositivity rate was highest in the 2 years old goats (13.04%), while no seropositivity was detected in 1-year-old goats. The highest seropositivity rate amongst the study sites was found in Birebir with 36.6%, whereas none of the goats were seropositive examined in Çiftliksarıca (April) and Yeşilçit. Sixteen (8.98%) out of 178 non-aborted goats were detected as seropositive, while there was no seropositivity in the 6 goats with abortion history. Neospora caninum seroprevalence was not found to be statistically significant with regards to the age groups of the goats and aborting or non-aborting status(P>0.05), on the other hand, the results showed statistically significant (P<0.001) with regards to the research sites. This is the first serological report on N.caninum in goats of Mesudiye district in Ordu province.

Anahtar Kelimeler

Neospora caninum, neosporosis, goat, Ordu–Mesudiye, seroprevalence, ELISA

Proje Numarası

FEB 2013/32-YÜLTEP

Kaynakça

• Al-Majali AM, Jawasreh KI, Talafha HA, Talafha AQ. Neosporosis in Sheep and Different Breeds of Goats from Southern Jordan: Prevalence and Risk Factors Analysis. Am J Anim Vet Sci. 2008; 3: 47-52.
• Anastasia D, Elias P, Nikolaos P, Charilaos K, Nektarios G. Toxoplasma gondii and Neospora caninum seroprevalence in dairy sheep and goats mixed stock farming. Vet Parasitol. 2013; 198: 387-390.
• Bartova E, Sedlak K. Toxoplasma gondii and Neospora caninum antibodies in goats in the Czech Republic. Vet Med-Czech. 2012; 57: 111-114.
• Björkman C, Uggla A. Serological diagnosis of Neospora caninum infection. Int J Parasitol. 1999; 29: 1497-1507.
• Bowman DD, Lynn RC, Eberhard ML. Georgis Parasitology for Veterinarians. Elsevier Science, USA, 2003.
• Cayvaz M, Karatepe M. Niğde Yöresi Keçilerinde Neospora caninum’un Seroprevalansı. Kafkas Univ Vet Fak Derg. 2011; 17: 935-939.
• Cobadiova, A., Reiterova, K., Derdakova, M., Spilovska, S., Turcekova, L., Hviscova, I. Hisira, V. Toxoplasma gondii, Neospora caninum and tick-transmitted bacterium Anaplasma phagocytophilum infections in one selected goat farm in Slovakia. Acta Parasitol 2013; 58: 541-546.
• Czopowicz M, Kaba J, Szalu´s-Jordanow O, Nowicki M, Witkowski L, Frymus T. Seroprevalence of Toxoplasma gondii and Neospora caninum infections in goats in Poland. Vet Parasitol. 2011; 178: 339–341.
• Dubey JP. Review of Neospora caninum and neosporosis in animals. Korean J Parasitol. 2003; 41: 1-16.
• Dubey JP, Carpenter JL, Speer CA, Topper MJ, Uggla A. Newly recognized fatal protozoon disease of dogs. J Am Vet Med Assoc. 1988; 192: 1269-1285.
• Dubey JP, Schares G, Ortega-Mora LM. Epidemiology and Control of Neosporosis and Neospora caninum. Clin Microbiol Rev. 2007; 20: 323-367.
• Faria EB, Gennari SM, Pena HFJ, Athayde ACR, Silva MLCR, Azevedo SS. Prevalence of anti- Toxoplasma gondii and anti-Neospora caninum antibodies in goats slaughtered in the public slaughterhouse of Patos city, Paraı’ba State, Northeast region of Brazil. Vet Parasitol. 2007; 149:126-129.
• Figliuolo LPC, Rodrigues AAR, Viana RB, Aguiar DM, Kasai N, Gennari SM. Prevalence of anti-Toxoplasma gondii and anti-Neospora caninum antibodies in goat from São Paulo State, Brazil. Small Ruminant Res. 2004; 55: 29-32.
• Iovu A, Györke A, Mircean V, Gavrea R, Cozma V. Seroprevalence of Toxoplasma gondii and Neospora caninum in dairy goats from Romania. Vet Parasitol 2012; 186: 470-474.
• McAllister MM, Dubey JP, Lindsay DS, Jolley WR, Wills RA, McGuire AM. Dogs are definitive hosts of Neospora caninum. Int J Parasitol. 1998; 28: 1473-1478.
• Moreno B, Collantes-Fernandez E, Villa A, Navarro A, Regidor-Cerrillo J, Ortega-Mora LM. Occurrence of Neospora caninum and Toxoplasma gondii infections in ovine and caprine abortions. Vet Parasitol. 2012; 187: 312-318.
• Naguleswaran A, Hemphill A, Rajapakse RPVJ. Sager H. Elaboration of a crude antigen ELISA for serodiagnosis of caprine neosporosis: validation of the test by detection of Neospora caninum-specific antibodies in goats from Sri Lanka. Vet Parasitol. 2004; 126: 257-262.
• Sevgili M, Çimtay İ, Keskin O. Şanlıurfa Yöresindeki Keçilerde Neospora caninum Enfeksiyonunun Seroprevalansı. Türkiye Parazitol Derg. 2003; 27: 249-251.
• Şuteu O, Paştiu A, Györke A, Avram E, Cozma V. Molecular detection of Neospora caninum in slaughtered goat kids from Romania. Sci Parasitol. 2013; 14: 43-46.
• Tembue AASM, Ramos RAN, Sousa TR, Albuquerque AR, Costa AJ, Meunier IMJ, Faustino MAG, Alves LC. Serological survey of Neospora caninum in small ruminants from Pernambuco State, Brazil. Rev Bras Parasitol Vet. 2011; 20: 246-248.
• Topazio JP, Weber A, Camillo G, Vogel FF, Machado G, Ribeiro A, Moura AB, Lopes LS, Tonin AA, Solda NM, Braunig P, Silva AS. Seroprevalence and risk factors for Neospora caninum in goats in Santa Catarina state, Brazil. Rev Bras Parasitol Vet. 2014; 23: 360-366.
• Unzaga JM, More G, Bacigalupe D, Rambeaud M, Pardini L, Dellarupe A, Felice LD, Gos ML, Venturini MC. Toxoplasma gondii and Neospora caninum infections in goat abortions from Argentina. Parasitol Int. 2014; 63: 865-867.
• Utuk AE, Simsek S, Piskin FC, Balkaya I. Detection of Neospora caninum IgG Antibodies in Goats in Elazig, Erzurum and Kırsehir Provinces of Turkey. Isr J Vet Med. 2011; 66: 157-160.
• Uzeda RS, Pinheiro AM, Fernandez SY, Ayres MCC, Gondim LFP, Almeida MAO. Seroprevalence of Neospora caninum in dairy goats from Bahia, Brazil. Small Ruminant Res. 2007; 70: 257–259.

Toplam 24 adet kaynakça vardır.

Ayrıntılar

Kaynak Göster

Cited By

Seroprevalence of <i>Neospora caninum</i> in Goats from Korkuteli District of Antalya

Turkish Journal of Parasitology https://doi.org/10.4274/tpd.galenos.2022.07269

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