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Phytochemical screening and in vitro biological activity of Amaranthus viridis growing in Northern Cyprus Skip to content Yıl 2024, Cilt: 11 Sayı: 3, 592 – 603, 29.08.2024
Melis Sümengen Özdenefe , Fikret Büyükkaya Kayış , Ümit Haydar Erol , Aysun Mercimek Takcı
Öz In this study, phenolic and non-phenolic metabolites in different parts of A. viridis (from the Turkish Republic of Northern Cyprus) were characterized with reversed-phase high-performance liquid chromatography (RP-HPLC) with diode array detector and high-performance liquid chromatography (HPLC), respectively. In total, approximately twenty-five phenolic compounds including quercetin, chrysin, t-Ferulic acid, and sinapic acid as the most abundant secondary metabolites were identified. On the other hand, four organic acids as non-phenolic compounds quantitatively predominant were identified for the first time in A. viridis extracts. The seed and flower extract showed strong ferric-reducing capacity, radical scavenging activity for DPPH˙, phosphomolybdenum assay, metal chelating, and α-amylase inhibition activity by in vitro assays. Our results suggest that A. viridis widely used in the human diet in Cyprus is a source of numerous metabolites showing antioxidant and antibacterial potential.
Anahtar Kelimeler Amaranthus viridis, Antioxidant potential, Northern Cyprus, Organic acid, RP-HPLC
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Indian Journal of Pharmacology, 50(3), 130-138. http://dx.doi.org/10.4103/ijp.IJP_77_18 Mareshvaran, U.R., Murugan, L., Salvamani, S., Sharma, M., Okechukwu, P.N., & Gunasekaran, B. (2020). In vitro Inhibitory potential of Amaranthus viridis against alpha-amylase for diabetes and the antioxidant activity. Malaysian Journal of Biochemistry & Molecular Biolology, 2, 127 – 134. Naeem, Z., Jabeen, K., & Iqbal, S. (2022). Management of ochratoxigenic fungi by phytochemicals of Amaranthus viridis L. Journal of Animal and Plant Sciences, 32(4), 1136-1142. http://doi.org/10.36899/JAPS.2022.4.0518 Olarewaju, O.A., Alashi, A.M., Taiwo, K.A., Oyedele, D., Adebooye, O.C., & Aluko, R.E. (2018). Influence of nitrogen fertilizer micro-dosing on phenolic content, antioxidant, and anticholinesterase properties of aqueous extracts of three tropical leafy vegetables. Journal of Food Biochemistry, 1-13. https://doi.org/10.1111/jfbc.12566 Oluwagunwa, O.A., Alashi, A.M., & Aluko, R.E. (2021). Inhibition of the in vitro activities of α-amylase and pancreatic lipase by aqueous extracts of Amaranthus viridis, Solanum macrocarpon and Telfairia occidentalis leaves. Frontiers in Nutrition, 8, 1 17. https://doi.org/10.3389/fnut.2021.772903 Owuso, E., Ahorlu, M.M., Afutu, E., Akumwena, A., & Asare, G.A. (2021). Antimicrobial activity of selected medicinal plants from a Sub-Saharan African Country against bacterial pathogens from post operative wound infections. Medical Sciences, 9(2), 23. https://doi.org/10.3390/medsci9020023 Oyaizu, M. (1986). Studies on product of browning reaction – Antioxidative activities of products of browning reaction prepared from glucose amine. The Japanese Journal of Nutrition and Dietetics, 44(6), 307–315. Pl@ntNet. (2024, Marc 22). Amaranthus viridis L. https://identify.plantnet.org/k-world-flora/species/Amaranthus%20viridis%20L./data Popoola, O.O. (2022). Phenolic compounds composition and in vitro antioxidant activity of Nigerian Amaranthus viridis seed as affected by autoclaving and germination. Measurement: Food, 6, 1-7. https://doi.org/10.1016/j.meafoo.2022.100028 Pulipati, S., Babu, P.S., & Narasu, M.L. (2014). Phytochemical and pharmacological potential of Amaranthus viridis L. International Journal of Phytomedicine, 6(3), 322-326. Reyad-ul-Ferdous, Md., Shahjahan, D. M. S., Tanvir, S., & Mukti, M. (2015). Present biological status of potential medicinal plant of Amaranthus viridis: A comprehensive review. American Journal of Clinical and Experimental Medicine, 3(5-1), 12-17. http://dx.doi.org/10.11648/j.ajcem.s.2015030501.13 Rose, A.L., Starlin, M., Wilsy, I., & Appavoo, R. (2021). Antibacterial activity of Amaranthus viridis L. International Journal of Innovative Research in Technology, 7(11), 420-422. Sadia, S., Mashwani, Z.U.R., Amin, H., Shedayi, A.A., Zhang, J-T., Bai, X., Nayyar, B.G., & Mazari, P. (2016). Qualitative and quantitative phytochemical analysis and antioxidant potential of Amaranthus Viridis L. from Pakistan. Proceedings of 54th The IIER International Conference, Beijing, China, 13th January 2016, ISBN: 978-93-82702-35-1, 10-16. Sarker, U., & Oba, S. (2019). Nutraceuticals, antioxidant pigments, and phytochemicals in the leaves of Amaranthus spinosus and Amaranthus viridis weedy species. Sci. Rep., 9:20413, 1-10. https://doi.org/10.1038/s41598-019-50977-5 Sarker, U., & Oba, S. (2020). Phenolic profiles and antioxidant activities in selected drought‑tolerant leafy vegetable amaranth. Scientific Reports, 10:18287, 1-11. https://doi.org/10.1038/s41598-020-71727-y Sarker, U., Hossain, M., & Oba, S. (2020). Nutritional and antioxidant components and antioxidant capacity in green morph Amaranthus leafy vegetable. Scientific Reports, 10:1336, 1-10. https://doi.org/10.1038/s41598-020-57687-3 Sharma, S., & Vig, A.P. (2013). Evaluation of in vitro antioxidant properties of methanol and aqueous extracts of Parkinsonia aculeata L. leaves. The Scientific World Journal, 1, 2–7. http://doi.org/10.1155/2013/604865 Stanković, M.S. (2011). Total phenolic content, flavonoid concentration and antioxidant activity of Marrubium peregrinum L. extracts. Kragujevac Journal of Science, 33, 63–72. Sunday, E.A., Gift, W.P., & Boobondah, W.J. (2021). Phytochemistry and antioxidant activity of Amaranthus viridis L (Green leaf). World Journal of Advanced Research and Reviews, 12(02), 306–314. https://doi.org/10.30574/wjarr.2021.12.2.0468 Swarnakumari, S., Mohan, S., Sasikala, M., & Umapoorani, T. (2021). Comparative studies on Amaranthus viridus and Amaranthus spinosus. International Journal of Pharmaceutical Sciences and Research, 12(10), 5618-5623. http://dx.doi.org/10.13040/IJPSR.0975-8232.12(10).5618-23 Torres, M.P., Carlos, L.A., Pedrosa, M.W., Silva, A.P.C.M., Silva, E.C., & Ferraz, L.C.L. (2018). Profile of phytochemistry and antioxidant activity of sorrel in function of organic fertilization and density of plantio. Bioscience Journal, 35(3), 775-783. Ucan Turkmen, F., & Mercimek Takci, H.A. (2018). Ultraviolet-C and ultraviolet-B lights effect on black carrot (Daucus carota ssp. sativus) juice. Journal of Food Measurement and Characterization, 12, 1038-1046. Zahir, S., Pal, T.K., Sengupta, A., Biswas, S., Bar, S., & Bose, S. (2021). Determination of lethal concentration fifty (LC50) of whole plant ethanolic extract of Amaranthus Viridis, Cynodon Dactylon & Aerva Sanguinolenta on Zebrafish (Danio Rerio) embryos. International Journal of Pharmaceutical Sciences and Research, 12(4), 2394-2404. http://dx.doi.org/10.13040/IJPSR.0975-8232.12(4).2394-04 Zhang, Q-W., Lin, L-G., & Ye, W-C. (2018). Techniques for extraction and isolation of natural products: a comprehensive review. Chinese Medicine, 13(20), 1 26. https://doi.org/10.1186/s13020-018-0177-x Zaware, O.R., Wakchaure, R.S., Tambe, A.V., & Lokhande, R.P. (2022). Antioxidant activity of Amaranthus Viridis Linn. International Journal of Advanced Research in Science, Communication and Technology, 2(5), 664-670. http://dx.doi.org/10.48175/IJARSCT-4880 Zengin, G., Sarikurkcu, C., Aktumsek, A., & Ceylan. R. (2014). Sideritis galatica Bornm.: A source of multifunctional agents for the management of oxidative damage, Alzheimer’s and diabetes mellitus. Journal of Functional Foods, 11, 538 547. https://doi.org/10.1016/j.jff.2014.08.011 Zong, Y., Li, J., Sun, W., Liu, G., Lu, J., & Shan, G. (2016). Determination of succinic acid in desvenlafaxine succinate by high performance ion-exclusion chromatography and high performance ion-exchange chromatography. Chinese Journal of Chromatography, 34(2), 189-193. https://doi.org/10.3724/SP.J.1123.2015.08017
Phytochemical screening and in vitro biological activity of Amaranthus viridis growing in Northern Cyprus Yıl 2024, Cilt: 11 Sayı: 3, 592 – 603, 29.08.2024
Melis Sümengen Özdenefe , Fikret Büyükkaya Kayış , Ümit Haydar Erol , Aysun Mercimek Takcı
Öz In this study, phenolic and non-phenolic metabolites in different parts of A. viridis (from the Turkish Republic of Northern Cyprus) were characterized with reversed-phase high-performance liquid chromatography (RP-HPLC) with diode array detector and high-performance liquid chromatography (HPLC), respectively. In total, approximately twenty-five phenolic compounds including quercetin, chrysin, t-Ferulic acid, and sinapic acid as the most abundant secondary metabolites were identified. On the other hand, four organic acids as non-phenolic compounds quantitatively predominant were identified for the first time in A. viridis extracts. The seed and flower extract showed strong ferric-reducing capacity, radical scavenging activity for DPPH˙, phosphomolybdenum assay, metal chelating, and α-amylase inhibition activity by in vitro assays. Our results suggest that A. viridis widely used in the human diet in Cyprus is a source of numerous metabolites showing antioxidant and antibacterial potential.
Anahtar Kelimeler Amaranthus viridis, Antioxidant potential, Northern Cyprus, Organic acid, RP-HPLC
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Indian Journal of Pharmacology, 50(3), 130-138. http://dx.doi.org/10.4103/ijp.IJP_77_18 Mareshvaran, U.R., Murugan, L., Salvamani, S., Sharma, M., Okechukwu, P.N., & Gunasekaran, B. (2020). In vitro Inhibitory potential of Amaranthus viridis against alpha-amylase for diabetes and the antioxidant activity. Malaysian Journal of Biochemistry & Molecular Biolology, 2, 127 – 134. Naeem, Z., Jabeen, K., & Iqbal, S. (2022). Management of ochratoxigenic fungi by phytochemicals of Amaranthus viridis L. Journal of Animal and Plant Sciences, 32(4), 1136-1142. http://doi.org/10.36899/JAPS.2022.4.0518 Olarewaju, O.A., Alashi, A.M., Taiwo, K.A., Oyedele, D., Adebooye, O.C., & Aluko, R.E. (2018). Influence of nitrogen fertilizer micro-dosing on phenolic content, antioxidant, and anticholinesterase properties of aqueous extracts of three tropical leafy vegetables. Journal of Food Biochemistry, 1-13. https://doi.org/10.1111/jfbc.12566 Oluwagunwa, O.A., Alashi, A.M., & Aluko, R.E. (2021). Inhibition of the in vitro activities of α-amylase and pancreatic lipase by aqueous extracts of Amaranthus viridis, Solanum macrocarpon and Telfairia occidentalis leaves. Frontiers in Nutrition, 8, 1 17. https://doi.org/10.3389/fnut.2021.772903 Owuso, E., Ahorlu, M.M., Afutu, E., Akumwena, A., & Asare, G.A. (2021). Antimicrobial activity of selected medicinal plants from a Sub-Saharan African Country against bacterial pathogens from post operative wound infections. Medical Sciences, 9(2), 23. https://doi.org/10.3390/medsci9020023 Oyaizu, M. (1986). Studies on product of browning reaction – Antioxidative activities of products of browning reaction prepared from glucose amine. The Japanese Journal of Nutrition and Dietetics, 44(6), 307–315. Pl@ntNet. (2024, Marc 22). Amaranthus viridis L. https://identify.plantnet.org/k-world-flora/species/Amaranthus%20viridis%20L./data Popoola, O.O. (2022). Phenolic compounds composition and in vitro antioxidant activity of Nigerian Amaranthus viridis seed as affected by autoclaving and germination. Measurement: Food, 6, 1-7. https://doi.org/10.1016/j.meafoo.2022.100028 Pulipati, S., Babu, P.S., & Narasu, M.L. (2014). Phytochemical and pharmacological potential of Amaranthus viridis L. International Journal of Phytomedicine, 6(3), 322-326. Reyad-ul-Ferdous, Md., Shahjahan, D. M. S., Tanvir, S., & Mukti, M. (2015). Present biological status of potential medicinal plant of Amaranthus viridis: A comprehensive review. American Journal of Clinical and Experimental Medicine, 3(5-1), 12-17. http://dx.doi.org/10.11648/j.ajcem.s.2015030501.13 Rose, A.L., Starlin, M., Wilsy, I., & Appavoo, R. (2021). Antibacterial activity of Amaranthus viridis L. International Journal of Innovative Research in Technology, 7(11), 420-422. Sadia, S., Mashwani, Z.U.R., Amin, H., Shedayi, A.A., Zhang, J-T., Bai, X., Nayyar, B.G., & Mazari, P. (2016). Qualitative and quantitative phytochemical analysis and antioxidant potential of Amaranthus Viridis L. from Pakistan. Proceedings of 54th The IIER International Conference, Beijing, China, 13th January 2016, ISBN: 978-93-82702-35-1, 10-16. Sarker, U., & Oba, S. (2019). Nutraceuticals, antioxidant pigments, and phytochemicals in the leaves of Amaranthus spinosus and Amaranthus viridis weedy species. Sci. Rep., 9:20413, 1-10. https://doi.org/10.1038/s41598-019-50977-5 Sarker, U., & Oba, S. (2020). Phenolic profiles and antioxidant activities in selected drought‑tolerant leafy vegetable amaranth. Scientific Reports, 10:18287, 1-11. https://doi.org/10.1038/s41598-020-71727-y Sarker, U., Hossain, M., & Oba, S. (2020). Nutritional and antioxidant components and antioxidant capacity in green morph Amaranthus leafy vegetable. Scientific Reports, 10:1336, 1-10. https://doi.org/10.1038/s41598-020-57687-3 Sharma, S., & Vig, A.P. (2013). Evaluation of in vitro antioxidant properties of methanol and aqueous extracts of Parkinsonia aculeata L. leaves. The Scientific World Journal, 1, 2–7. http://doi.org/10.1155/2013/604865 Stanković, M.S. (2011). Total phenolic content, flavonoid concentration and antioxidant activity of Marrubium peregrinum L. extracts. Kragujevac Journal of Science, 33, 63–72. Sunday, E.A., Gift, W.P., & Boobondah, W.J. (2021). Phytochemistry and antioxidant activity of Amaranthus viridis L (Green leaf). World Journal of Advanced Research and Reviews, 12(02), 306–314. https://doi.org/10.30574/wjarr.2021.12.2.0468 Swarnakumari, S., Mohan, S., Sasikala, M., & Umapoorani, T. (2021). Comparative studies on Amaranthus viridus and Amaranthus spinosus. International Journal of Pharmaceutical Sciences and Research, 12(10), 5618-5623. http://dx.doi.org/10.13040/IJPSR.0975-8232.12(10).5618-23 Torres, M.P., Carlos, L.A., Pedrosa, M.W., Silva, A.P.C.M., Silva, E.C., & Ferraz, L.C.L. (2018). Profile of phytochemistry and antioxidant activity of sorrel in function of organic fertilization and density of plantio. Bioscience Journal, 35(3), 775-783. Ucan Turkmen, F., & Mercimek Takci, H.A. (2018). Ultraviolet-C and ultraviolet-B lights effect on black carrot (Daucus carota ssp. sativus) juice. Journal of Food Measurement and Characterization, 12, 1038-1046. Zahir, S., Pal, T.K., Sengupta, A., Biswas, S., Bar, S., & Bose, S. (2021). Determination of lethal concentration fifty (LC50) of whole plant ethanolic extract of Amaranthus Viridis, Cynodon Dactylon & Aerva Sanguinolenta on Zebrafish (Danio Rerio) embryos. International Journal of Pharmaceutical Sciences and Research, 12(4), 2394-2404. http://dx.doi.org/10.13040/IJPSR.0975-8232.12(4).2394-04 Zhang, Q-W., Lin, L-G., & Ye, W-C. (2018). Techniques for extraction and isolation of natural products: a comprehensive review. Chinese Medicine, 13(20), 1 26. https://doi.org/10.1186/s13020-018-0177-x Zaware, O.R., Wakchaure, R.S., Tambe, A.V., & Lokhande, R.P. (2022). Antioxidant activity of Amaranthus Viridis Linn. International Journal of Advanced Research in Science, Communication and Technology, 2(5), 664-670. http://dx.doi.org/10.48175/IJARSCT-4880 Zengin, G., Sarikurkcu, C., Aktumsek, A., & Ceylan. R. (2014). Sideritis galatica Bornm.: A source of multifunctional agents for the management of oxidative damage, Alzheimer’s and diabetes mellitus. Journal of Functional Foods, 11, 538 547. https://doi.org/10.1016/j.jff.2014.08.011 Zong, Y., Li, J., Sun, W., Liu, G., Lu, J., & Shan, G. (2016). Determination of succinic acid in desvenlafaxine succinate by high performance ion-exclusion chromatography and high performance ion-exchange chromatography. Chinese Journal of Chromatography, 34(2), 189-193. https://doi.org/10.3724/SP.J.1123.2015.08017 Toplam 41 adet kaynakça vardır.
Ayrıntılar Birincil Dil İngilizce Konular Bitki Biyokimyası, Mikrobiyoloji (Diğer) Bölüm Makaleler Yazarlar Melis Sümengen Özdenefe NEAR EAST UNIVERSITY 0000-0003-0804-9557 Cyprus
Fikret Büyükkaya Kayış ADIYAMAN UNIVERSITY 0000-0002-3890-1635 Türkiye
Ümit Haydar Erol KILIS 7 ARALIK UNIVERSITY 0000-0001-6126-5844 Türkiye
Aysun Mercimek Takcı KILIS 7 ARALIK UNIVERSITY 0000-0002-3388-1153 Türkiye
Erken Görünüm Tarihi 6 Ağustos 2024 Yayımlanma Tarihi 29 Ağustos 2024 Gönderilme Tarihi 24 Ocak 2024 Kabul Tarihi 20 Nisan 2024 Yayımlandığı Sayı Yıl 2024 Cilt: 11 Sayı: 3
Kaynak Göster APA Sümengen Özdenefe, M., Büyükkaya Kayış, F., Erol, Ü. H., Mercimek Takcı, A. (2024). Phytochemical screening and in vitro biological activity of Amaranthus viridis growing in Northern Cyprus. International Journal of Secondary Metabolite, 11(3), 592-603.
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