THE PHARMACEUTICAL BOTANICAL STUDIES ON THE ENDEMIC Asperula pestalozzae Boiss. (RUBIACEAE)

Yıl 2024, Cilt: 13 Sayı: 2, 86 – 99, 30.07.2024

Ayla Kaya Kader Kayiş

https://doi.org/10.18036/estubtdc.1382598

Öz

Bu çalışmada endemik Asperula pestalozzae Boiss. (Rubiaceae) farmasötik botanik açısından araştırıldı. Morfolojik, anatomik, mikromorfolojik, palinolojik ve kimyasal (antioksidan aktivite ve toplam fenolik madde miktarı) özellikleri ilk kez ayrıntılı olarak rapor edildi. A. pestalozzae’nin genişletilmiş açıklamaları ve görüntüleri verildi. Anatomik açıklama ayrıntılı olarak sunuldu ve fotoğraflarla desteklendi. Gövde genellikle köşeli veya dairesel şekillidir ve yaprak tek yüzlüdür. Tüy ve polen mikromorfolojisi taramalı elektron mikroskobu (SEM) ile incelendi. A. pestalozzae’nin polen taneleri monad, radyal simetri, izopolar ve heksakolpattır (bazen heptakolpattır). Antioksidan aktiviteyi belirlemek için DPPH ve ABTS radikalleri kullanıldı. DPPH %50 inhibisyon konsantrasyonu (IC50) değeri standart gallik asit için 0,0011±0,0002 mg/ml, A. pestalozzae ekstraktı için 0,134±0,017 mg/ml olarak bulunmuştur. 0,1 mg/ml ekstraktta 0,022±0,36 Mm TEAC aktivitesi; 10 mg/ml ekstraktta 1.437±0.51 mM TEAC aktivitesi belirlendi. Toplam fenolik maddelerin miktarı 53 mg gallik asit eşdeğeri (GAE) olarak hesaplandı.

Anahtar Kelimeler

anatomi, antioksidan aktivite, Asperula, morfoloji, palinoloji

Teşekkür

Yazarlar olarak, kimyasal analizlerdeki yardımlarından dolayı Afyonkarahisar Sağlık Bilimleri Üniversitesi'nden Doç. Dr. Fatih GÖGER'e ve Anadolu Üniversitesi Bitki, İlaç ve Bilimsel Araştırma Uygulama ve Araştırma Merkezi'nden Dr. Biyolog Şennur GÖRGÜLÜ'ye SEM çalışmasına verdiği destekten dolayı teşekkür ederiz.

Kaynakça

• [1] Öztürk M. Asperula anatolica (Rubiaceae), a new species from south-east Anatolia, Turkey. Turkish Journal of Botany 2013; 37 (1): 46-54.
• [2] Soza VL, Olmstead RG. Molecular systematics of tribe Rubieae (Rubiaceae): evolution of major clades, development of leaf-like whorls, and biogeography. Taxon 2010; 59 (3): 755–771.
• [3] Ehrendorfer F, Barfuss MHJ, Manen JF, Schneeweiss GM. Phylogeny, character evolution and spatiotemporal diversification of the species rich and world-wide distributed tribe Rubieae (Rubiaceae). PLOS ONE 2018; 13 (12): 1-26.
• [4] Minareci E. The revision of the section Thlipthisa (Griseb.) Ehrend. of the genus Asperula L. (Rubiaceae) spreading in Turkey. PhD, Celal Bayar University, Manisa, Turkey, 2007.
• [5] Özgen U, Coşkun M. Morphological and anatomical studies on Asperula taurina L. subsp. caucasica (Pobed.) Ehrend. (Rubiaceae). Ankara Üniversitesi Eczacılık Fakültesi Dergisi 1999; 28 (2): 71-83.
• [6] Martins D, Nunez CV. Secondary metabolites from Rubiaceae species. Molecules 2015; 20 (7): 13422-13495.
• [7] Özgen U, Şener SÖ, Badem M, Seçinti H, Hatipoğlu SD, Gören AC, Kazaz C, Palaska E. Evaluation of HPLC, phytochemical, anticholinesterase and antioxidant profiles of the aerial parts of Asperula taurina subsp. caucasica. Ankara Üniversitesi Eczacılık Fakültesi Dergisi 2018; 42 (1): 1-13.
• [8] Akdeniz S. Pollen morphology of some taxa belonging to the genera Asperula L., Galium L. (Rubiaceae) distributed in Turkey. MSc, Bitlis Eren University, Bitlis, Turkey, 2019.
• [9] Iurchenko NS, Ilyina TV, Kovaleva AM, Toryanik EL, Kulish IA. The antihypoxic and sedative activity of the dry extract from Asperula odorata L. Pharmacognosy Communications 2015; 5 (4): 233-236.
• [10] Web Address of BİZİM BİTKİLER: https://www.bizimbitkiler.org.tr (accessed: 04.02.2024)
• [11] Davis PH. Flora of Turkey and the East Aegean Islands. Volume 7: Edinburgh University Press, Edinburgh, 1982, pp.722-767.
• [12] Sezer O, Öztürk D, Ocak A, Koyuncu O. Flora of Phrygian Valley (Mountain Phrygia/Turkey). Biological Diversity and Conservation 2017; 10 (3), 163-183.
• [13] Erdem CB The flora and ethnobotany of Abbaslık village (Bilecik). MSc, Hacettepe University, Ankara, Turkey, 2018.
• [14] Gücel S. Morphology, anatomy and cytology of critically endangered endemic Asperula daphneola from, West Anatolia, Turkey. Journal of Environmental Biology 2015; 36 (1): 129-132.
• [15] Minareci E, Yıldız K. Karyotype characterization of Turkish taxa of the genus Asperula L.-section Thlipthisa (Rubiaceae). Indian Society of Genetics & Plant Breeding 2011; 71 (1), 49-54.
• [16] Minareci E, Ergönül B, Kayalar H, Kalyoncu F. Chemical compositions and antioxidant activities offive endemic Asperula taxa. Archives of Biological Sciences 2011; 63 (3): 537-543.
• [17] Halimi M, Nasrabadi M. Essential oil composition and antioxidant activity of aerial parts of Asperula oppositifolia collected from Darkesh, Iran. Journal of Medicinal Plants Research 2015; 9 (46): 1118-1122.
• [18] Güvenalp Z, Demirezer LÖ. Flavonol glycosides from Asperula arvensis L. Turkish Journal of Chemistry 2005; 29 (2): 163-169.
• [19] Özgen U, Kazaz C, Seçen H, Coşkun M. Phytochemical studies on the underground parts of Asperula taurina subsp. caucasica. Turkish Journal of Chemistry 2006; 30 (1): 15-20.
• [20] Kırmızıbekmez H, Bardakcı H, Masullo M, Kamburoğlu Ö, Eryılmaz G, Akaydın G, Yeşilada E, Piacente S. Flavonol glycosides and iridoids from Asperula lilaciflora. Helvetica Chimica Acta 2014; 97 (11): 1571-1576.
• [21] Kırmızıbekmez H, Tiftik K, Kusz N, Orban-Gyapai O, Zomborszki ZP, Hohmann J. Three new iridoid glycosides from the aerial parts of Asperula involucrata. Chemistry & Biodiversity 2017; 14 (3): 1-7.
• [22] Kalyoncu F, Minareci E, Minareci O. Antimicrobial activity of five endemic Asperula species from Turkey. Iranian Journal of Pharmaceutical Research 2009; 8 (4): 263-268.
• [23] Loizzo MR, Saabb AM, Tundisa R, Menichinia F, Bonesia M, Piccoloa V, Statti GA, De Cindioc B, Houghtond PJ, Menichinia F. In vitro inhibitory activities of plants used in Lebanon traditional medicine against angiotensin converting enzyme (ACE) and digestive enzymes related to diabetes. Journal of Ethnopharmacology 2008; 119 (1): 109-116.
• [24] Baytop A. English-Turkish Botanical Manual, İstanbul University Press, İstanbul, 1998, pp.359-375.
• [25] Metcalfe CR, Chalk L. Anatomy of the Dicotyledons, Vol 2: Oxford University Press, London, 1950, pp.759-776.
• [26] Walker JW, Doyle JA. The bases of angiosperm phylogeny: polynology. Annals of the Missouri Botanical Garden 1975, 62, pp.664-723.
• [27] Zhao M, Ito Y, Tu P. Isolation of a novel flavanone 6-glucoside from the flowers of Carthamus tinctorium (Honghua) by high-speed counter-current chromatography. Journal of Chromatography A 2005; 1090: 193–196.
• [28] Büyüktuncel E. Main spectrophotometric methods for the determination of total phenolic content and antioxidant capacity. Marmara Pharmaceutical Journal 2013; 17: 93-103.
• [29] Kosar M, Göger F, Can Baser KH. In vitro antioxidant properties and phenolic composition of Salvia virgata Jacq. from Turkey. J. Agric. Food Chem. 2008; 56 (7): 2369-2374.
• [30] Singleton VL, Orthofer R, Lamuela-Raventós RM (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of folinciocalteu reagent. Meth. Enzymol. 1999; 299: 152-178.
• [31] Kumarasamy Y, Byres M, Cox PJ, Jaspars M, Nahar L, Sarker SD. Screening seeds of some Scottish plants for free radical scavenging activity. Phytother. Res. 2007; 21 (7): 615-621.
• [32] Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. 1999; 26 (9-10): 1231-123.
• [33] Minareci E, Yıldız K. Asperula pseudochlorantha var. antalyensis comb. et stat. nov. (Rubiaceae). Annales Botanici Fennici 2010; 47: 121-128.
• [34] Şahin B, Sağıroğlu M, Başer B. A new Asperula L. (Rubiaceae) species from gypsum steppes of Çankırı province in Turkey. Turkish Journal of Botany 2021; 45 (2): 243-252.

THE PHARMACEUTICAL BOTANICAL STUDIES ON THE ENDEMIC Asperula pestalozzae Boiss. (RUBIACEAE)

Yıl 2024, Cilt: 13 Sayı: 2, 86 – 99, 30.07.2024

Ayla Kaya Kader Kayiş

https://doi.org/10.18036/estubtdc.1382598

Öz

In this study, endemic Asperula pestalozzae Boiss. (Rubiaceae) was investigated in aspects of pharmaceutical botany. The morphological, anatomical, micromorphological, palynological, and chemical (antioxidant activity and total amount of phenolic substance) characteristics were reported in detail for the first time. Expanded descriptions and images of A. pestalozzae were given. The anatomical description was presented in detail and supported by photographs. The stem is usually angular or orbicular shaped and the leaf is monofacial. Its trichome and pollen micromorphology were examined with a scanning electron microscope (SEM). The pollen grains of A. pestalozzae are monad, radial symmetry, isopolar, and hexacolpate (sometimes heptacolpate). DPPH and ABTS radicals were used to determine antioxidant activity. The DPPH 50% inhibition concentration (IC50) value was found to be 0.0011±0.0002 mg/ml for standard gallic acid and 0.134±0.017 mg/ml for A. pestalozzae extract. 0.1 mg/ml extract calculated 0.022±0.36 Mm TEAC activity; 1.437±0.51 mM TEAC activity was determined in 10 mg/ml extract. The total amount of phenolic substances was calculated as 53 mg gallic acid equivalent (GAE).

Anahtar Kelimeler

Anatomy, antioxidant activity, Asperula, morphology, palynology

Teşekkür

The authors would like to thank Doç. Dr. Fatih GÖGER of Afyonkarahisar Health Sciences University for his assistance with chemical analysis, as well as the Anadolu University Plant, Medicine, and Scientific Research Application and Research Center and Dr. Bio. Şennur GÖRGÜLÜ for her assistance with the SEM study.

Kaynakça

• [1] Öztürk M. Asperula anatolica (Rubiaceae), a new species from south-east Anatolia, Turkey. Turkish Journal of Botany 2013; 37 (1): 46-54.
• [2] Soza VL, Olmstead RG. Molecular systematics of tribe Rubieae (Rubiaceae): evolution of major clades, development of leaf-like whorls, and biogeography. Taxon 2010; 59 (3): 755–771.
• [3] Ehrendorfer F, Barfuss MHJ, Manen JF, Schneeweiss GM. Phylogeny, character evolution and spatiotemporal diversification of the species rich and world-wide distributed tribe Rubieae (Rubiaceae). PLOS ONE 2018; 13 (12): 1-26.
• [4] Minareci E. The revision of the section Thlipthisa (Griseb.) Ehrend. of the genus Asperula L. (Rubiaceae) spreading in Turkey. PhD, Celal Bayar University, Manisa, Turkey, 2007.
• [5] Özgen U, Coşkun M. Morphological and anatomical studies on Asperula taurina L. subsp. caucasica (Pobed.) Ehrend. (Rubiaceae). Ankara Üniversitesi Eczacılık Fakültesi Dergisi 1999; 28 (2): 71-83.
• [6] Martins D, Nunez CV. Secondary metabolites from Rubiaceae species. Molecules 2015; 20 (7): 13422-13495.
• [7] Özgen U, Şener SÖ, Badem M, Seçinti H, Hatipoğlu SD, Gören AC, Kazaz C, Palaska E. Evaluation of HPLC, phytochemical, anticholinesterase and antioxidant profiles of the aerial parts of Asperula taurina subsp. caucasica. Ankara Üniversitesi Eczacılık Fakültesi Dergisi 2018; 42 (1): 1-13.
• [8] Akdeniz S. Pollen morphology of some taxa belonging to the genera Asperula L., Galium L. (Rubiaceae) distributed in Turkey. MSc, Bitlis Eren University, Bitlis, Turkey, 2019.
• [9] Iurchenko NS, Ilyina TV, Kovaleva AM, Toryanik EL, Kulish IA. The antihypoxic and sedative activity of the dry extract from Asperula odorata L. Pharmacognosy Communications 2015; 5 (4): 233-236.
• [10] Web Address of BİZİM BİTKİLER: https://www.bizimbitkiler.org.tr (accessed: 04.02.2024)
• [11] Davis PH. Flora of Turkey and the East Aegean Islands. Volume 7: Edinburgh University Press, Edinburgh, 1982, pp.722-767.
• [12] Sezer O, Öztürk D, Ocak A, Koyuncu O. Flora of Phrygian Valley (Mountain Phrygia/Turkey). Biological Diversity and Conservation 2017; 10 (3), 163-183.
• [13] Erdem CB The flora and ethnobotany of Abbaslık village (Bilecik). MSc, Hacettepe University, Ankara, Turkey, 2018.
• [14] Gücel S. Morphology, anatomy and cytology of critically endangered endemic Asperula daphneola from, West Anatolia, Turkey. Journal of Environmental Biology 2015; 36 (1): 129-132.
• [15] Minareci E, Yıldız K. Karyotype characterization of Turkish taxa of the genus Asperula L.-section Thlipthisa (Rubiaceae). Indian Society of Genetics & Plant Breeding 2011; 71 (1), 49-54.
• [16] Minareci E, Ergönül B, Kayalar H, Kalyoncu F. Chemical compositions and antioxidant activities offive endemic Asperula taxa. Archives of Biological Sciences 2011; 63 (3): 537-543.
• [17] Halimi M, Nasrabadi M. Essential oil composition and antioxidant activity of aerial parts of Asperula oppositifolia collected from Darkesh, Iran. Journal of Medicinal Plants Research 2015; 9 (46): 1118-1122.
• [18] Güvenalp Z, Demirezer LÖ. Flavonol glycosides from Asperula arvensis L. Turkish Journal of Chemistry 2005; 29 (2): 163-169.
• [19] Özgen U, Kazaz C, Seçen H, Coşkun M. Phytochemical studies on the underground parts of Asperula taurina subsp. caucasica. Turkish Journal of Chemistry 2006; 30 (1): 15-20.
• [20] Kırmızıbekmez H, Bardakcı H, Masullo M, Kamburoğlu Ö, Eryılmaz G, Akaydın G, Yeşilada E, Piacente S. Flavonol glycosides and iridoids from Asperula lilaciflora. Helvetica Chimica Acta 2014; 97 (11): 1571-1576.
• [21] Kırmızıbekmez H, Tiftik K, Kusz N, Orban-Gyapai O, Zomborszki ZP, Hohmann J. Three new iridoid glycosides from the aerial parts of Asperula involucrata. Chemistry & Biodiversity 2017; 14 (3): 1-7.
• [22] Kalyoncu F, Minareci E, Minareci O. Antimicrobial activity of five endemic Asperula species from Turkey. Iranian Journal of Pharmaceutical Research 2009; 8 (4): 263-268.
• [23] Loizzo MR, Saabb AM, Tundisa R, Menichinia F, Bonesia M, Piccoloa V, Statti GA, De Cindioc B, Houghtond PJ, Menichinia F. In vitro inhibitory activities of plants used in Lebanon traditional medicine against angiotensin converting enzyme (ACE) and digestive enzymes related to diabetes. Journal of Ethnopharmacology 2008; 119 (1): 109-116.
• [24] Baytop A. English-Turkish Botanical Manual, İstanbul University Press, İstanbul, 1998, pp.359-375.
• [25] Metcalfe CR, Chalk L. Anatomy of the Dicotyledons, Vol 2: Oxford University Press, London, 1950, pp.759-776.
• [26] Walker JW, Doyle JA. The bases of angiosperm phylogeny: polynology. Annals of the Missouri Botanical Garden 1975, 62, pp.664-723.
• [27] Zhao M, Ito Y, Tu P. Isolation of a novel flavanone 6-glucoside from the flowers of Carthamus tinctorium (Honghua) by high-speed counter-current chromatography. Journal of Chromatography A 2005; 1090: 193–196.
• [28] Büyüktuncel E. Main spectrophotometric methods for the determination of total phenolic content and antioxidant capacity. Marmara Pharmaceutical Journal 2013; 17: 93-103.
• [29] Kosar M, Göger F, Can Baser KH. In vitro antioxidant properties and phenolic composition of Salvia virgata Jacq. from Turkey. J. Agric. Food Chem. 2008; 56 (7): 2369-2374.
• [30] Singleton VL, Orthofer R, Lamuela-Raventós RM (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of folinciocalteu reagent. Meth. Enzymol. 1999; 299: 152-178.
• [31] Kumarasamy Y, Byres M, Cox PJ, Jaspars M, Nahar L, Sarker SD. Screening seeds of some Scottish plants for free radical scavenging activity. Phytother. Res. 2007; 21 (7): 615-621.
• [32] Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. 1999; 26 (9-10): 1231-123.
• [33] Minareci E, Yıldız K. Asperula pseudochlorantha var. antalyensis comb. et stat. nov. (Rubiaceae). Annales Botanici Fennici 2010; 47: 121-128.
• [34] Şahin B, Sağıroğlu M, Başer B. A new Asperula L. (Rubiaceae) species from gypsum steppes of Çankırı province in Turkey. Turkish Journal of Botany 2021; 45 (2): 243-252.

Toplam 34 adet kaynakça vardır.

Ayrıntılar

Kaynak Göster

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